RT-PCR protocols / edited by Nicola King. — 2nd ed. — New York : Springer, 2010. – (58.17/M592/v.630)

Contents

    Contents
    
    Preface v
    Contributors ix
    PART I THE RT-PCR DETECTIVE: HUNTING DOWN THE BEST METHOD
    1 Single Cell RT-PCR on Mouse Embryos: A General Approach for Developmental Biology
    2 Poly(A) cDNA Real-Time PCR for Indicator Gene Measurement in Cancer
    3 Transcriptome Profiling of Host-Microbe Interactions by Differential Display RT-PCR
    4 Quantitative RT-PCR Methods for Mature microRNA Expression Analysis
    5 Detection of Influenza A Virus Neuraminidase and PB2 Gene Segments by One Step Reverse Transcription Polymerase Chain Reaction 65
    6 Detection and Identification of CD46 Splicing Isoforms by Nested RT-PCR 83
    7 Simultaneous Detection of Bluetongue Virus RNA， Internal Control GAPDH mRNA， and External Control Synthetic RNA by Multiplex Real-Time PCR
    8 Detection of West Nile Viral RNA from Field-Collected Mosquitoes in Tropical Regions by Conventional and Real-Time RT-PCR 109
    9 Detection of Antisense RNA Transcripts by Strand-Specific RT-PCR 125
    10 RT-PCR Amplification and Cloning of Large Viral Sequences 139
    PART II THE RT-PCR MATHEMATICIAN: ASSESSING GENE AND RNA EXPRESSION
    11 One-Step RT-LATE-PCR for mRNA and Viral RNA Detection and Quantification
    12 Changes in Gene Expression of Caveolin-1 After Inflammatory Pain Using Quantitative Real-Time PCR
    13 Real-Time Quantitative Reverse Transcriptase Polymerase Chain Reaction 199
    14 The Use of Comparative Quantitative RT-PCR to Investigate the Effect of Cysteine Incubation on GPx1 Expression in Freshly Isolated Cardiomyocytes
    15 The Renaissance of Competitive PCR as an Accurate Tool for Precise Nucleic Acid Quantification
    PART III THE RT-PCR MASTER CHEF: FINDING THE RIGHT INGREDIENTS
    16 Skeletal Muscle RNA Extraction in Preparation for RT-PCR
    17 Reverse Transcription of the Ribonucleic Acid: The First Step in RT-PCR Assay
    18 Primer Design for RT-PCK 271
    19 Hot Start PCR 301
    20 Real-time RT-PCR for Automated Detection ofHIV-1 RNA During Blood Donor Screening 319
    Index