G-quadruplex DNA : methods and protocols / edited by Peter Baumann. — New York : Humana Press, c2010. – (58.17/M592/v.608)

Contents

    Contents
    
    Preface
    Contributors
    1 G-Quadruplexes: From Guanine Gels to Chemotherapeutics
    2 Molecular Modeling and Simulation of G-Quadruplexes and Quadruplex-Ligand Complexes
    3 Computational Approaches to the Detection and Analysis of Sequences with Intramolecular G-Quadruplex Forming Potential
    4 Preparation of G-Quartet Structures and Detection by Native Gel Electrophoresis
    5 Biochemical Techniques for the Characterization of G-Quadruplex Structures: EMSA， DMS Footprinting， and DNA Polymerase Stop Assay
    6 Real-Time Observation of G-Quadruplex Dynamics Using Single-Molecule FRET Microscopy 81
    7 Sedimentation Velocity Ultracentrifugation Analysis for Hydrodynamic Characterization of G-Quadruplex Structures 97
    8 2-Aminopurine as a Probe for Quadruplex Loop Structures 121
    9 Assessing DNA Structures with 125I Radioprobing 137
    10 Monitoring the Temperature Unfolding of G-Quadruplexes by UV and Circular Dichroism Spectroscopies and Calorimetry Techniques 147
    11 Probing Telomeric G-Quadruplex DNA Structures in Cells with In Vitro Generated Single-Chain Antibody Fragments 159
    12 Detection of G-Quadruplexes in Cells and Investigation of G-Quadruplex Structure of d(T2AG3)4 in K+ Solution by a Carbazole Derivative: BMVC 183
    13 Isolation of G-Quadruplex DNA Using NMM-Sepharose Affinity Chromatography
    14 Quantifying Interactions Between G-Quadruplex DNA and Transition-Metal Complexes
    15 G4-FID: A Fluorescent DNA Probe Displacement Assay for Rapid Evaluation of Quadruplex Ligands 257
    Index 273