Methods in cell biology. v.91, Cilia : structure and motility / edited by Stephen M. King, Gregory J. Pazour. — Amsterdam : Academic Press, 2009. -- (58.1574/M592/V.91)

Contents

    CONTENTS
    
    Contributors
    Preface
    1. Cryo-Electron Microscope Tomography to Study Axonemal Organization
    I. Introduction and Rationale
    II. Methods and Materials
    III. Discussion
    References
    2. Electron Microscopic Imaging and Analysis of Isolated Dynein Particles
    I. Introduction
    II. Rationale
    III. Methods
    VI. Summary
    References
    3. Immunogold Labeling of Flagellar Components In Situ
    I. Introduction
    II. Methods for Immunogold Labeling of Flagellar Components
    III. Materials
    IV. Summary and Discussion
    References
    4. Scanning Electron Microscopy to Examine Cells and Organs
    I. Introduction
    II. Materials
    III. Metal-Impregnation Method for Scanning EM Analysis of Mouse Kidneys (modified from McManus et al.， 1993)
    IV. Method for Scanning EM Visualization of Mouse Nodal Cilia
    V. Summary
    References
    5. X-ray Fiber Diffraction Studies on Flagellar Axonemes
    I. Introduction
    II. Rationale
    III. Methods
    IV. Results and Discussion
    V. Summary
    References
    6. Markers for Neuronal Cilia
    I. Introduction
    II. Immunoenzymatic Labeling of Neuronal Cilia in Brain Sections
    III. Immunofluorescent Labeling of Neuronal Cilia in Brain Sections
    IV. Visualization of Neuronal Cilia In Vitro
    V. Summary
    References
    7. Immunofluorescence Staining of Ciliated Respiratory Epithelial Cells
    I. Introduction
    II. Antibodies
    III. Materials and Methods
    IV. Methods
    V. Discussion
    References
    8. Immunoprecipitation to Examine Protein Complexes
    I. Introduction
    II. General Procedure
    III. Controls for Immunoprecipitation Assays
    IV. Summary
    References
    9. Tandem Affinity Purification of Ciliopathy-Associated Protein Complexes
    I. Introduction
    II. Methods
    III. Data Evaluation
    IV. Outlook
    References
    10. Crosslinking Methods: Purification and Analysis of Crosslinked Dynein Products
    I. Chemical Crosslinking
    II. Identification of Interaction Partners
    III. Summary
    References
    11. Analysis of the Ciliary/Flagellar Beating of Chlamydomonas
    I. Introduction
    II. Rationale
    III. Techniques
    IV. Future Directions
    References
    12. Assays of Cell and Axonemal Motility in Chlamydomonas reinhardtii
    I. Introduction
    II. Motility Assessments in Live Chlamydomonas Cells
    III. In Vitro Reactivation of Axonemal Beating
    IV. Microtubule Sliding by Axonemal Dynein
    References
    13. High-Speed Digital Imaging of Ependymal Cilia in the Murine Brain
    I. Introduction
    II. Materials and Equipment
    III. Methods
    IV. Discussion
    V. Summary
    References
    14. Observation of Nodal Cilia Movement and Measurement of Nodal Flow
    I. Introduction and Historical Background
    II. Observation of Nodal Cilia Motility in Mouse Embryo
    III. Observation of Nodal Flow in Mouse Embryo
    IV. Observation of Nodal Cilia Motility and Nodal Flow in Rabbit Embryo
    V. Discussion
    References
    15. Modification of Mouse Nodal Flow by Applying Artificial Flow
    I. Introduction
    II. Solutions
    III. Experimental Setup
    IV. Methods
    V. Discussion
    References
    16. Measuring Cilium-Induced Ca2+ Increases in Cultured Renal Epithelia
    I. Introduction
    II. Methods
    III. Materials
    IV. Summary/Conclusion
    References