Neuroproteomics : methods and protocols / edited by Andrew K. Ottens and Kevin K. W. Wang. — New York : Humana ; London : Springer, 2009. – (58.17/M592/v.566)

Contents

    Contents
    
    Preface
    Contributors
    1. The Methodology of Neuroproteomics
    PART I DISEASE MODELS IN NEUROPROTEOMICS
    2. Modeling Cerebral Ischemia in Neuroproteomics 25
    3. Clinical and Model Research of Neurotrauma 41
    4. Neuroproteomic Methods in Spinal Cord Injury
    5. Modeling Substance Abuse for Applications in Proteomics
    6. Protein Aggregate Characterization in Models of Neurodegenerative Disease
    PART II SUB-PROTEOME SEPARATIONS AND NEUROPROTEOMIC ANALYSIS
    7. Sub-Proteome Processing: Isolation of Neuromelanin Granules from the Human Brain
    8. Proteomic Analysis of Protein Phosphorylation and Ubiquitination in Alzheimer's Disease 109
    9. Proteomics Identification of Carbonylated and HNE-Bound Brain Proteins in Alzheimer's Disease
    10. Mass Spectrometric Identification of In Vivo Nitrotyrosine Sites in the Human Pituitary Tumor Proteome 137
    11. Improved Enrichment and Proteomic Analysis of Brain Proteins with Signaling Function by Heparin Chromatography 165
    12. Calmodulin-Binding Proteome in the Brain 181
    PART III NEUROPROTEOMIC METHODOLOGY AND BIOINFORMATICS
    13. Separation of the Neuroproteome by Ion Exchange Chromatography
    14. iTRAQ-Based Shotgun Neuroproteomics
    15. Methods in Drug Abuse Neuroproteomics: Methamphetamine Psychoproteome 217
    16. Shotgun Protein Identification and Quantification by Mass Spectrometry in Neuroproteomics 229
    PART IV BIOFLUID ANALYSIS AND CLINICAL TRANSLATION
    17. Identification of Glycoproteins in Human Cerebrospinal Fluid 263
    18. Mass Spectrometric Analysis of Body Fluids for Biomarker Discovery 277
    19. Traumatic Brain Injury Biomarkers: From Pipeline to Diagnostic Assay Development 293
    20. Translation of Neurological Biomarkers to Clinically Relevant Platforms 303
    Index