Reverse chemical genetics : methods and protocols / edited by Hisashi Koga. — New York : Humana Press, c2009. – (58.17/M592/v.577)

Contents

    Contents
    
    Preface
    Contributors
    PART I THE IMPORTANCE OF FULLY-CHARACTEKIZED GENOMIC RESOURCES AND INFORMATICS FOR REVERSE CHEMICAL GENETICS
    1. ORFeome Cloning
    2. Systematic Cloning of an ORFeome Using the Gateway System
    3. High-Throughput Construction of ORF Clones for Production of the Recombinant Proteins
    4. Computational Overview of GPCR Gene Universe to Support Reverse Chemical Genomics Study
    5. High-Performance Gene Expression Module Analysis Tool and Its Application to Chemical Toxicity Data
    6. Human Protein Reference Database and Human Proteinpedia as Discovery Tools for Systems Biology
    PART II MODIFICATION OF cDNA RESOURCES UTILIZED FOR REVERSE CHEMICAL GENETICS
    7. High-Throughput Production of the Recombinant Proteins Expressed in Escherichia coli Utilizing cDNA Resources
    8. An Insect Cell-Free System for Recombinant Protein Expression Using cDNA Resources
    9. Recombinant Protein Production by a Kaiko-Baculovirus System
    10. Pulse-Chase Experiment for the Analysis of Protein Stability in Cultured Mammalian Cells by Covalent Fluorescent Labeling of Fusion Proteins
    PART III THE REFINEMENT OF ESTABLISHED METHODS BY RECENT INNOVATIONS
    11. Application of 2D-DIGE in Cancer Proteomics Toward Personalized Medicine
    12. Fully Automated Two-Dimensional Electrophoresis System for High-Throughput Protein Analysis
    13. Large-Scale Whole Mount In Situ Hybridization of Mouse Embryos
    14. Identification of the Specific Binding Proteins of Bioactive Small Compound Using Affinity Resins
    PART IV POTENTIAL APPLICABLE TECHNIQUES FOR REVERSE CHEMICAL GENETICS
    15. Protein Microarrays: Effective Tools for the Study of Inflammatory Diseases
    16. Preparation of Highly Sensitive Protein Array Using Reactive Polymer
    17. High-Throughput SPR Biosensor
    18. Drug Discovery Through Functional Screening in the Drosophila Heart
    19. Permeable Cell Assay: A Method for High-Throughput Measurement of Cellular ATP Synthetic Activity
    20. Novel Methodology for Immobilization of Biomolecules on the Surface of a Photoresponsible Polymer Containing Azobenzene Moiety
    Index