Advanced protocols in oxidative stress II / edited by Donald Armstrong. — Totowa, N.J. : Humana, 2010. – (58.17/M592/v.594)

Contents

    Contents
    
    Preface
    Contributors
    PART I REACTIVE OXYGEN AND NITROGEN TECHNIQUES
    1 Current Status of Measuring Oxidative Stress..
    2 pO2 and ROS/RNS Measurements in the Microcirculation in Hypoxia
    3 Nitrate Reductase Activity of Mitochondrial Aldehyde Dehydrogenase (ALDH-2) as a Redox Sensor for Cardiovascular Oxidative Stress
    4 Identification of ROS Using Oxidized DCFDA and Flow-Cytometry
    5 Measurement of 8-Isoprostane in Exhaled Breath Condensate
    6 Electron Paramagnetic Resonance Oximetry and Redoximetry
    7 Measurement of Mitochondrial Membrane Potential and Proton Leak 107
    8 Determination of Erythrocyte Fragility as a Marker of Pesticide-Induced Membrane Oxidative Damage 123
    9 Using N，N，N'，N'-tetramethyl-p-phenylenediamine (TMPD) to Assay Cyclooxygenase Activity In Vitro 129
    10 Structural and Functional Changes in the Insulin Molecule Produced by Oxidative Stress 141
    11 Multiphoton Redox Ratio Imaging for Metabolic Monitoring In Vivo 155
    12 Using Fluorescence-Activated Flow Cytometry to Determine Reactive Oxygen Species Formation and Membrane Lipid Peroxidation in Viable Boar Spermatozoa
    13 Lipofuscin: Detection and Quantification by Microscopic Techniques 173
    PART II ANTIOXIDANT TECHNOLOGY AND APPLICATION
    14 OXY-SCORE: A Global Index to Improve Evaluation of Oxidative Stress by Combining Pro- and Antioxidant Markers 197
    15 Cupric Ion Reducing Antioxidant Capacity Assay for Antioxidants in Human Serum and for Hydroxyl Radical Scavengers 215
    16 Analysis of Antioxidant Activities in Vegetable Oils and Fat Soluble Vitamins and Biofactors by the PAO-SO Method
    17 Measuring Antioxidant Capacity Using the ORAC and TOSC Assays.
    18 Assessing the Neuroprotective Effect of Antioxidant Food Factors by Application of Lipid-Derived Dopamine Modification Adducts 263
    19 LIBS-Based Detection of Antioxidant Elements: A New Strategy 275
    20 A Method for Evaluation of Antioxidant Activity Based on Inhibition of Free Radical-Induced Erythrocyte Hemolysis 287
    21 Design and Synthesis of Antioxidant α-Lipoic Acid Hybrids 297
    22 Characterization of the Antioxidant Properties of Pentaerithrityl Tetranitrate (PETN)-Induction of the Intrinsic Antioxidative System Heme Oxygenase-1 (HO-1) 311
    23 Direct Determination of Tissue Aminothiol， Disulfide， and Thioether Levels Using HPLC-ECD with a Novel Stable Boron-Doped Diamond Working Electrode : 327
    24 Activation of Erythrocyte Plasma Membrane Redox System Provides a Useful Method to Evaluate Antioxidant Potential of Plant Polyphenols 341
    25 Antioxidant Activity of Biotransformed Sex Hormones Facilitated by Bacillus Stearothermophilus 349
    26 Separation of Phenylpropanoids and Evaluation of Their Antioxidant Activity 357
    PART III GENE EXPRESSION
    27 Generation of Antioxidant Adenovirus Gene Transfer Vectors Encoding CuZnSOD， MnSOD， and Catalase
    28 HPLC Purification of Adenoviral Vectors 395
    29 Mapping of Oxidative Stress Response Elements of the Caveolin-1 Promoter 409
    PART IV BIOSTATISTICS
    30 Meta-Analysis: Drawing Conclusions When Study Results Vary 427
    Index