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Handbook of cryo-preparation methods for electron microscopy / edited by Annie Cavalier, Daniele Spehner, Bruno M. Humbel. — Boca Raton : CRC Press, c2009. – (58.1714/H236) |
Contents
CONTENTS
Introduction
Abbreviations
Chapter 1 Vitreous Water
Part I - Cryo-Fixation Methods
Chapter 2 Slam-Freezing, Metal-Mirror Freezing
Chapter 3 Plunge-Freezing (Holey Carbon Method)
Chapter 4 Controlled Vitrification
Chapter 5 BAL-TEC HPM 010 High-Pressure Freezing Machine
Chapter 6 High-Pressure Freezing LEICA EMPACT
Part II - Cryo-Electron Microscopy
Chapter 7 Frozen-Hydrated Macromolecules for Structural Analysis
Chapter 8 Two-Dimensional Crystals
Chapter 9 Cryo-Negative Staining
Chapter 10 Vitrification of Dynamic Microtubules
Chapter 11 CEMOVIS: Cryo-Electron Microscopy of Vitreous Sections
Chapter 12 Cryo-Electron Tomography
Part III - Low-Temperature Embedding
Chapter 13 Freeze-Substitution
Chapter 14 Cryo-Fixation, Freeze-Substitution, Rehydration and Tokuyasu Cryo- Sectioning
Chapter 15 Freeze-Drying and Embedding of Biological Material
Part IV - Freeze-Fracture and Metal Shadowing
Chapter 16 The Shadow of Hydrated Biological Specimens
Chapter 17 Cryo-Fracture of Self-Assembled Systems in Organic Solvent
Part V - Analysis
Chapter 18 Progressive Lowering of Temperature for Immunolabeling and in situ Hybridization
Chapter 19 Cryo-Sectioning According to Tokuyasu
Chapter 20 Cryo-Preparation Procedures for Elemental lmaging by SIMS and EFTEM
Chapter 21 Correlative Light and Electron Microscopy
Chapter 22 SDS-digested Freeze-Fracture Replica Labeling (SDS-FRL)
Chapter 23 Immunolabeling of Ultrathin Sections with Enlarged 1 nm Gold or QDots
Chapter 24 3-D Electron Tomography of Cells and Organelles
Final Considerations
Glossary
Index