Proteomics : introduction to methods and applications / edited by Agnieszka Kraj, Jerzy Silberring. — Hoboken, N.J. : Wiley, c2008. – (58.17421/P967pt)

Contents

    CONTENTS
    
    Contributors
    Preface
    1 Introduction to Proteomics and Strategy for Protein Identification
    2 Sample Preparation
    3 Chromatographic Separation Methods
    3.1 Liquid Chromatography Linked to Mass Spectrometry
    3.2 Multidimensional Liquid Chromatography Combined with Mass Spectrometry
    4 Electrophoretic Separation Methods
    4.1 One-Dimensional Gel Electrophoresis
    4.2 Two-Dimensional Gel Electrophoresis
    5 Capillary Electrophoresis
    6 Protein Blotting
    7 Introduction to Protein and Peptide Mass Spectrometry
    7.1 Electrospray Ionization
    7.2 Matrix-Assisted Laser Desorption/Ionization
    7.3 Imaging Mass Spectrometry
    7.4 Fragmentation and Sequencing in Proteomics
    7.5 Protein Identification by Mass Spectrometry
    7.6 Chemical Derivatization for
    8 Protein Modifications
    9 Microfluidics
    10 Proteomics
    10.1 Quantitative Proteomics
    10.2 Structural Proteomics
    10.3 Functional Proteomics
    10.4 Clinical Proteomics
    11 Bioinformatics
    Appendix A: Laboratory Exercises
    Appendix B: Data Tables
    Appendix C: Internet Resources
    Index