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Proteomics : introduction to methods and applications / edited by Agnieszka Kraj, Jerzy Silberring. — Hoboken, N.J. : Wiley, c2008. – (58.17421/P967pt) |
Contents
CONTENTS
Contributors
Preface
1 Introduction to Proteomics and Strategy for Protein Identification
2 Sample Preparation
3 Chromatographic Separation Methods
3.1 Liquid Chromatography Linked to Mass Spectrometry
3.2 Multidimensional Liquid Chromatography Combined with Mass Spectrometry
4 Electrophoretic Separation Methods
4.1 One-Dimensional Gel Electrophoresis
4.2 Two-Dimensional Gel Electrophoresis
5 Capillary Electrophoresis
6 Protein Blotting
7 Introduction to Protein and Peptide Mass Spectrometry
7.1 Electrospray Ionization
7.2 Matrix-Assisted Laser Desorption/Ionization
7.3 Imaging Mass Spectrometry
7.4 Fragmentation and Sequencing in Proteomics
7.5 Protein Identification by Mass Spectrometry
7.6 Chemical Derivatization for
8 Protein Modifications
9 Microfluidics
10 Proteomics
10.1 Quantitative Proteomics
10.2 Structural Proteomics
10.3 Functional Proteomics
10.4 Clinical Proteomics
11 Bioinformatics
Appendix A: Laboratory Exercises
Appendix B: Data Tables
Appendix C: Internet Resources
Index