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Meiosis. v. 1, Molecular and genetic methods / edited by Scott Keeney. — Dordrecht ; London : Springer, c2009. – (58.17/M592/v.557) |
Contents
Contents
Preface
Contributors
SECTION I: GENETIC METHODS FOR STUDYING MEIOTIC RECOMBINATION AND CHROMOSOME DYNAMICS
1 Interaction of Genetic and Environmental Factors in Saccharomyces cerevisiae Meiosis: The Devil is in the Details
2 Optimizing Sporulation Conditions for Different Saccharomyces cerevisiae Strain Backgrounds 21
3 Modulating and Targeting Meiotic Double-Strand Breaks in Saccharomyces cerevisiae 27
4 Methods for Analysis of Crossover Interference in Saccharomyces cerevisiae
5 Measurement of Spatial Proximity and Accessibility of Chromosomal Loci in Saccharomyces cerevisiae Using Cre/loxP Site-Specific Recombination.
6 Genetic Analysis of Meiotic Recombination in Schizosaccharomyces pombe
7 Analysis of Meiotic Recombination in Caenorhabditis elegans
8 Visual Markers for Detecting Gene Conversion Directly in the Gametes of Arabidopsis thaliana
SECTION II: MOLECULAR ANALYSIS OF RECOMBINATION AND PROTEIN-DNA INTERACTIONS DURING MEIOSIS
9 Gel Electrophoresis Assays for Analyzing DNA Double-Strand Breaks in Saccharomyces cerevisiae at Various Spatial Resolutions
10 Genome-Wide Mapping of Meiotic DNA Double-Strand Breaks in Saccharomyces cerevisiae
11 Detection of Meiotic DNA Breaks in Mouse Testicular Germ Cells
12 End-Labeling and Analysis of Spo11-Oligonucleotide Complexes in Saccharomyces cerevisiae
13 Detection of SPO11-Oligonucleotide Complexes from Mouse Testes
14 Stabilization and Electrophoretic Analysis of Meiotic Recombination
Intermediates in Saccharomyces cerevisiae
15 Using Schizosaccharomyces pombe Meiosis to Analyze DNA Recombination Intermediates 235
16 Analysis of Chromatin Structure at Meiotic DSB Sites in Yeasts 253
17 Analysis of Protein-DNA Interactions During Meiosis by Quantitative Chromatin Immunoprecipitation (qChIP)
18 Genome-Wide High-Resolution Chromatin Immunoprecipitation of Meiotic Chromosomal Proteins in Saccharomyces cerevisiae
19 Parallel Detection of Crossovers and Noncrossovers in Mouse Germ Cells 305
20 Analysis of Meiotic Recombination Products from Human Sperm 323
Index
