Protocols for in vitro cultures and secondary metabolite analysis of aromatic and medicinal plants / edited by S. Mohan Jain and Praveen K. Saxena. — New York, NY : Humana, c2009. – (58.17/M592/v.547)

Contents

    Contents
    
    Preface
    Contributors
    PART I: MICROPROPAGATION AND CHEMICAL ANALYSIS
    1. Establishment of Adventitious Root Cultures of Echinacea purpurea for the Production of Caffeic Acid Derivatives
    2. In Vitro Propagation of Rauwolfia serpentina Using Liquid Medium， Assessment of Genetic Fidelity of Micropropagated Plants， and Simultaneous Quantitation of Reserpine， Ajmaline， and Ajmalicine
    3. Medicinal Properties， In Vitro Protocols and Secondary Metabolite Analyses of Scots Pine
    4. Saussurea medusa Cell Suspension Cultures for Flavonoid Production
    5. Large-scale In Vitro Multiplication of Crataeva nurvala
    6. Bilberry In Vitro Protocols and Analyses of Phenolic Compounds
    7. In Vitro Propagation of Two Tuberous Medicinal Plants: Holostemma ada-kodien and Ipomoea mauritiana
    8. In Vitro Production of Gymnemic Acid from Gymnema sylvestre (Retz) R. Br. Ex Roemer and Schultes Through Callus Culture Under Abiotic Stress Conditions
    9. Establishment of Plant Regeneration and Cryopreservation System from Zygotic Embryo-Derived Embryogenic Cell Suspension Cultures of Ranunculus kazusensis
    10. In Vitro Culture and Secondary Metabolite Isolation in Bryophytes
    11. Micropropagation and In Vitro Conservation of Vanilla (Vanilla planifolia Andrews)
    12. Protocol for In Vitro Regeneration and Marker Glycoside Assessment in Swertia chirata Buch-Ham 139
    13. Protocols for Establishment of an In Vitro Collection of Medicinal Plants in the Genus Scutellaria 155
    14. Protocols for In Vitro Culture and Phytochemical Analysis of Phyllanthus Species (Euphorbiaceae)
    15. In Vitro Clonal Propagation of Asparagus racemosus， a High Value Medicinal Plant 179
    16. Micropropagation of Penthorum chinense Through Axillary Buds
    PART II: TRANSGENIC APPROACHES
    17. Spontaneous Plant Regeneration and Production of Secondary Metabolites from Hairy Root Cultures of Centaurium erythraea Rafn 205
    18. Transgenic Hypericum perforatum 217
    19. Agrobacterium-Mediated Transformation of Ruta graveolens L
    20. Gene Expression Profiling in Taxus baccata L. Seedlings and Cell Cultures 249
    21. Catapol Production in Chinese Foxglove (Rehmannia glutinosa Libos.) Hairy Roots Transformed with Agrobacterium rhizogenes ATCC15834 263
    PART III: MOLECULAR MARKERS AND MICROSATELLITES
    22. Identification of Medicinal Plants and Plant Sequences: A Multiplexed MLPA Assay 277
    23. Isolation of Microsatellites from Catharanthus roseus (L.) G. Don Using Enriched Libraries 289
    PART IV: BIOTRANSFORMATION~ BIOREACTORS~ AND METABOLOMICS
    24. Production of Cinnamyl Glycosides in Compact Callus Aggregate Cultures of Rhodiola rosea Through Biotransformation of Cinnamyl Alcohol
    25. Spearmint Plantlet Culture System as a Means to Study Secondary Metabolism
    26. Bioreactor Production of Secondary Metabolites from Cell Cultures of Periwinkle and Sandalwood
    27. Camptothecin Production by In Vitro Cultures and Plant Regeneration in Ophiorrhiza Species
    28. Metabolomic Analysis of Ocotea odorifera Cell Cultures: A Model Protocol for Acquiring Metabolite Data
    29. The Production of 9-methoxycanthin-6-one from Callus Cultures of (Eurycoma longifolia Jack) Tongkat Ali 359
    PART V: ALTERED GRAVITY AND BIOTECHNOLOGY
    30. Plant Secondary Metabolism in Altered Gravity 373
    31. The Role of Biotechnology in the Production of the Anticancer Compound Podophyllotoxin
    Index 403