Transgenesis techniques : principles and protocols / edited by Elizabeth J. Cartwright. — 3rd ed. — New York, N.Y. : Humana Press, c2009. – (58.17/M592/v.561)

Contents

    Contents
    
    Preface
    Contributors
    PART I TRANSGENESIS IN VARIOUS MODEL SYSTEMS
    1. Transgenesis in Drosophila melanogaster
    2. Transgenesis in Caenorhabditis elegans
    3. Transgenesis in Zebrafish with the Tol2 Transposon System
    4. Generation of Transgenic Frogs
    5. Pronuclear DNA Injection for the Production of Transgenic Rats
    PART II TRANSGENESIS IN THE MOUSE
    6. Cell-Type-Specific Transgenesis in the Mouse 91
    7. Transgene Design and Delivery into the Mouse Genome: Keys to Success 105
    8. Overexpression Transgenesis in Mouse: Pronuclear Injection 111
    9. Gene-Targeting Vectors
    10. Gene Trap: Knockout on the Fast Lane
    11. Culture of Murine Embryonic Stem Cells
    12. Targeting Embryonic Stem Cells
    13. Generation of Chimeras by Microinjection
    14. Generation of Chimeras by Morula Aggregation
    15. Surgical Techniques for the Generation of mutant Mice
    16. Site-Specific Recombinases for Manipulation of the Mouse Genome
    17. Cre Transgenic Mouse Lines
    18. Large-Scale Mouse Mutagenesis
    19. Dedicated Mouse Production and Husbandry
    20. Biological Methods for Archiving and Maintaining Mutant Laboratory Mice. Part I: Conserving Mutant Strains
    21. Biological Methods for Archiving and Maintaining Mutant Laboratory Mice. Part II: Recover)， and Distribution of Conserved Mutant Strains
    Index