 |
Human embryogenesis : methods and protocols / edited by Julie Lafond, Cathy Vaillancourt. — New York : Humana press, 2009. – (58.17/M592/v.550) |
Contents
Contents
Preface
Contributors
SECTION I: INTRODUCTION: WHAT IS HUMAN EMBRYOGENESIS?
1. Human Embryogenesis: Overview
SECTION II: STUDYING EMBRYO IMPLANTATION: FROM TROPHOBLASTS TO EMBRYO
2. Differential Gene Expression in the Uterus and Blastocyst During the Reactivation of Embryo Development in a Model of Delayed Implantation 11
3. Coculture of Decidua and Trophoblast to Study Proliferation and Invasion
4. Isolation and Culture of Term Human Cytotrophoblast Cells and In Vitro Methods for Studying Human Cytotrophoblast Cells' Calcium Uptake 73
5. Endocrinology and Cell Signaling in Human Villous Trophoblast 89
6. Gestation-Induced Uterine Vascular Remodeling
SECTION III: STERIODOGENESIS AND REPRODUCTIVE SYSTEM
7. Placental and Fetal Steroidogenesis 127
8. Current Methods in Investigating the Development of the Female Reproductive System 137
9. A Revised and Improved Method for the Isolation of Seminiferous Tubule-Enriched Fractions that Preserves the Phosphorylated and Glycosylated Forms of Proteins
10. A Novel Technical Approach for the Measurement of Individual ACAT-1 and ACAT-2 Enzymatic Activity in the Testis 169
SECTION IV: FROM EMBRYO TO FETAL DEVELOPMENT
11. Genetic Dissection of Caenorhabditis elegans Embryogenesis Using RNA Interference and Flow Cytometry 181
12. Immune System: Maturation of Myeloid Cells 195
13. Functional Development of Human Fetal Gastrointestinal Tract 205
14. Renal and Cardiac Na+-K+-ATPase and Aconitase in a Rat Model of Fetal Programming
SECTION V: EMBRYOTOXICITY， IN VITRO FERTILIZATION， CLONING， AND ETHICS
15. Assessment of Embryotoxicity Using Mouse Embryo Culture 241
16. Fertilization In Vitro 251
17. Animal Cloning by Somatic Cell Nuclear Transfer 267
18. The Human Embryo: Ethical and Legal Aspects 281
Index 307
