Cell-based assays for high-throughput screening : methods and protocols / edited by Paul A. Clemons, Nicola J. Tolliday, and Bridget K. Wagner. -- Totowa, N.J. : Humana ; London : Springer [distributor], c2009. – (58.17/M592/v.486)

Contents

    Contents
    
    Preface
    Contributors
    1. Introduction: Cell-Based Assays for High-Throughput Screening 1
    2. High-Throughput Screening of Model Bacteria 13
    3. Cell-Based Assays to Probe the ERK MAP Kinase Pathway in Endothelial Cells 29
    4. Large-Scale Small-Molecule Screen Using Zebrafish Embryos
    5. Whole-Animal High-Throughput Screens: The C. elegans Model
    6. Whole-Organism Screening: Plants
    7. Fluorescence-Based Assays
    8. Reporter Gene Assays 10
    9. Screening for Chemical Inhibitors of Heterologous Proteins Expressed in Yeast Using a Simple Growth-Restoration Assay 125
    10. Assay for Isolation of Inhibitors of Her2-Kinase Expression
    11. High-Content Screening: Flow Cytometry Analysis
    12. High-Throughput Real-Time PCR for Detection of Gene-Expression Levels
    13. Interpretation of Uniform-Well Readouts
    14. Extracting Rich Information from Images
    Index