Antibody phage display : methods and protocols / edited by Robert Aitken. — 2nd ed. — Dordrecht ; London : Humana Press, c2009. – (58.17/M592/v.562)

Contents

    Contents
    
    Preface
    Contributors
    1 Antibody Phage Display: Overview of a Powerful Technology that Has Quickly Translated to the Clinic
    2 Design and Construction of Synthetic Phage-Displayed Fab Libraries
    3 Affinity Isolation of Antigen-Specific Circulating B Cells for Generation of Phage Display-Derived Human Monoclonal Antibodies
    4 Construction of Phage Antibody Repertoires from the Blood of West Nile Virus-Infected Donors
    5 Anti-J32 GP-I and Anti-prothrombin Antibodies Generated by Phage Display
    6 The Generation and Selection of Single-Domain， V Region Libraries from Nurse Sharks
    7 The Isolation of scFvs Against Small Target Molecules
    8 Display and Selection of scFv Antibodies on HEK-293T Cells
    9 Isolation of scFvs that Inhibit the NS3 Protease of Hepatitis C Virus by a Combination of Phage Display and a Bacterial Genetic Screen 115
    10 Guided Selection Methods Through Chain Shuffling
    11 Sequential Antigen Panning for Selection of Broadly Cross-Reactive HIV-1-Neutralizing Human Monoclonal Antibodies 143
    12 Selection of Antibodies Able to Rapidly Enter Mammalian Cells 155
    13 Identification of the Specificity of Isolated Phage Display Single-Chain Antibodies Using Yeast Two-Hybrid Screens 165
    14 Generation of Bispecific and Tandem Diabodies
    15 Intrabody Expression in Eukaryotic Cells
    16 High-Level Periplasmic Expression and Purification of scFvs 205
    17 Expression of Single-Chain Fv Fragments in E. coli Cytoplasm 215
    18 High-Level Expression of a Phage Display-Derived scFv in Pichia pastoris 225
    Index 237