DNA microarrays for biomedical research : methods and protocols / edited by Martin Dufva. — Totowa, N.J. : Humana, c2009. – (58.17/M592/v.529)

Contents

    Contents
    
    Preface
    Contributors
    1. Introduction to Microarray Technology
    2. Probe Design for Expression Arrays Using OligoWiz
    3. Comparative Genomic Hybridization: Microarray Design and Data Interpretation
    4. Design of Tag SNP Whole Genome Genotyping Arrays
    5. Fabrication of DNA Microarray
    6. Immobilization Chemistries
    7. Fabrication Using Contact Spotter.
    8. RNA Preparation and Characterization for Gene Expression Studies
    9. Gene Expression Analysis Using Agilent DNA Microarrays
    10. Target Preparation for Genotyping Specific Genes or Gene Segments
    11. Genotyping of Mutations in the Beta-Globin Gene Using Allele Specific Hybridization
    12. Microarray Temperature Optimization Using Hybridization Kinetics
    13. Whole-Genome Genotyping on Bead Arrays
    14. Genotyping Single Nucleotide Polymorphisms by Multiplex Minisequencing Using Tag-Arrays
    15. Resequencing Arrays for Diagnostics of Respiratory Pathogens
    16. Comparative Genomic Hybridization: DNA Preparation for Microarray Fabrication
    17. Comparative Genomic Hybridization: DNA Labeling， Hybridization and Detection 267
    18. Chromatin Immunoprecipitation Using Microarrays
    Index