Viral applications of green fluorescent protein : methods and protocols / edited by Barry W. Hicks. — Totowa, N.J. : Humana, c2009. –(58.17/M592/v.515)

Contents

    Contents
    
    1. Using Fluorescent Proteins to Study Poxvirus Morphogenesis
    2. Molecular Imaging of Human Embryonic Stem Cells
    3. Use of a GFP-PML-Expressing Cell Line as a Biosensor for Human Cytomegalovirus Infection
    4. Dual-Color Imaging of Tumor Angiogenesis
    5. Use of GFP to Analyze Morphology， Connectivity， and Function of Cells in the Central Nervous System 63
    6. Expression of EGFP by Adenovirus-Mediated Gene Transfer in the Central Nervous System
    7. Adenovirus-Mediated Delivery of Short Hairpin RNA (shRNA) Mediates Efficient Gene Silencing in Terminally Differentiated Cardiac Myocytes
    8. Microscopic Analysis of Adenoviral Decontamination Using GFP Adenovirus with Comparable Sensitivity to Flow Cytometry
    9. Production of Multicistronic HIV-1-Based Lentiviral Vectors
    10. Using an EGFPmeter to Evaluate the Lentiviral Vector Production: Tricks and Traps
    11. Use of a Macrophage-Tropic GFP-Tagged Human Immunodeficiency Virus Type 1 (HIV-1) to Study Viral Reservoirs
    12. GFP-Lentiviral Vectors Targeting for NeuroAIDS
    13. Construction of Cell-Type-Specific Promoter Lentiviruses for Optically Guiding Electrophysiological Recordings and for Targeted Gene Delivery
    14. Selective Protein Expression Within the CNS Using Hybrid Lentivirus
    15. Construction and Analysis of Alphaherpesviruses Expressing Green Fluorescent Protein
    16. The Use of Green Fluorescent Fusion Proteins to Monitor Herpes Simplex Virus Replication
    17. Modified Bovine Herpesvirus 1 for Protein Secretion
    18. Identification of Viral Peptide Fragments for Vaccine Development
    19. Use of GFP to Investigate Expression of Plant-Derived Vaccines
    20. Studies of the Role and Function of Barley Stripe Mosaic Virus Encoded Proteins in Replication and Movement Using GFP Fusions..
    21. Sendai Virus for Cancer Immunotherapy
    22. Poxvirus Tropism for Primary Human Leukocytes and Hematopoietic Cells
    23. Reverse Genetics of Nipah Virus to Probe Viral Pathogenicity
    24. Baculovirus-Mediated Gene Transfer into Mesenchymal Stem Cells
    Index