DNA and RNA profiling in human blood : methods and protocols / edited by Peter Bugert. — New York : Humana Press, c2009. – (58.17/M592/v.496)

Contents

    Contents
    
    Preface
    PART I DNA PROFILING FOR BLOOD CELL ANTIGENS
    1 PCR-ELISA for High-Throughput Blood Group Genotyping
    2 Single Base Extension in Multiplex Blood Group Genotyping
    3 Real-Time PCR Assays for High-Throughput Blood Group Genotyping
    4 Real-Time PCR Assays for High-Throughput Human Platelet Antigen Typing
    5 Multiplex ABO Genotyping by Minisequencing
    6 Multiplex Genotyping for Thrombophilia-Associated SNPs by Universal Bead Arrays
    7 Pyrosequencing of Toll-Like Receptor Polymorphisms of Functional Relevance
    8 Multiplex HLA-Typing by Pyrosequencing
    9 High-Throughput Multiplex HLA-Typing by Ligase Detection Reaction (LDR) and Universal Array (UA) Approach
    10 Medium- to High-Throughput SNP Genotyping Using VeraCode Microbeads
    11 The Use of Maternal Plasma for Prenatal RhD Blood Group Genotyping
    PART II RNA PROFILING IN BLOOD CELLS
    12 Nanoliter High-Throughput PCR for DNA and RNA Profiling
    13 RNA Stabilization of Peripheral Blood and Profiling by Bead Chip Analysis
    14 RNA Profiling in Peripheral Blood Cells by Fluorescent Differential Display PCR
    15 cDNA Amplification by SMART-PCR and Suppression Subtractive Hybridization (SSH)-PCR
    16 Transcript Profiling of Human Platelets Using Microarray and Serial Analysis of Gene Expression (SAGE)
    17 Genome-Wide Platelet RNA Profiling in Clinical Samples
    18 PCR-Based Amplification of Platelet mRNA Sequences Obtained From Small-Scale Platelet Samples
    19 MicroRNA Profiling of Megakaryocytes
    20 Serial Analysis of Gene Expression Adapted for Downsized Extracts (SAGE/SADE) Analysis in Reticulocytes
    21 Real-Time PCR Analysis for Blood Cell Lineage Specific Markers
    22 Monitoring the Immune Response Using Real-Time PCR
    Index