Candida albicans : methods and protocols / edited by Ronald L. Cihlar and Richard A. Calderone. — New York : Humana Press, c2009. – (58.17/M592/v.499)

Contents

    Contents
    
    Preface
    Contributors
    PART I IMMUNOGICAL METHODS
    1． Isolation of Dendritic Cells from Human Blood for In Vitro Interaction Studies with Fungal Antigens 3
    2. Detection and Quantitation of Antifungal SIgA Antibodies in Body Fluids 9
    3. Phagocytosis and Killing Assays for Candida Species
    4. Immunization Protocols for Use in Animal Models of Candidiasis 27
    PART II VIRULENCE AND BIOFILMS
    5. Penetration of Antifungal Agents Through Candida Biofilms 37
    6. Candida Biofilm Analysis in the Artificial Throat Using FISH 45
    7. Conditions for Optimal Candida Biofilm Development in Microtiter Plates
    PART III VIRULENCE MEASUREMENTS: IN VITRO， Ex VIVO， AND IN VIVO
    8． Animal Models of Candidiasis 65
    9． Candida albicans Gene Expression in an In Vivo Infection Model 77
    10． In Vitro and Ex Vivo Assays of Virulence in Candida albicans 85
    PART IV STRAIN TYPING AND IDENTIFICATION
    11． Biotyping of Candida albicans and Other Fungi by Yeast Killer Toxins Sensitivity
    12． DNA Fingerprinting Candida Species
    PART V GENOMICS AND PKOTEOMICS
    13． The Application of Tandem-Affinity Purification to Candida albicans 133
    14． Preparation of Samples for Proteomic Analysis of the Candida Albicans Cell Wall
    15． Reporter Gene Assays in Candida albicans 157
    16． Genetic Transformation of Candida albicans 169
    17． Large-Scale Gene Disruption Using the UAU1 Cassette
    PART VI APPENDIX
    18． Standard Growth Media and Common Techniques for Use with Candida Albicans
    Index