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Molecular embryology : methods and protocols / edited by Paul T. Sharpe and Ivor Mason. — 2nd ed. — New York : Humana Press, c2008. – (58.17/M592/v.461) |
Contents
Contents
PART I. THE MOUSE EMBRYO
1 The Mouse as a Developmental Mode
2 Culture of Postimplantation Mouse Embryos
3 Organ Culture in the Analysis of Tissue Interactions 23
4 Treatment of Mice with Retinoids In Vivo and In Vitro 31
5 Analysis of Skeletal Ontogenesis through Differential Staining of Bone and Cartilage
6 Cell Grafting and Labeling in Postimplantation Mouse Embryos 47
7 Production of Transgenic Rodents by the Microinjection of Cloned DNA into Fertilized One-Celled Eggs 71
8 Cre Recombinase Mediated Alterations of the Mouse Genome Using Embryonic Stem Cells
9 Gene Trapping in Mouse Embryonic Stem Cells 133
10 Application of lacZ Transgenic Mice to Cell Lineage Studies 149
11 Transgenic RNA Interference to Investigate Gene Function in the Mouse 165
12 Mouse Primordial Germ Cells: Isolation and In Vitro Culture 187
13 Gene Transfer to the Rodent Embryo by Retroviral Vectors 201
PART II. THE CHICKEN EMBRYO
14 The Avian Embryo: An Overview
15 Chick Embryos: Incubation and Isolation
16 New Culture 235
17 EC Culture: A Method to Culture Early Chick Embryos 255
18 Grafting Hensen's Node 265
19 Grafting of Somites 277
20 Microsurgical Manipulation of the Notochord 289
21 Transplantation of Avian Neural Tissue
22 Grafting of Apical Ridge and Polarizing Region 313
23 Tissue Recombinations in Collagen Gels
24 Quail-Chick Chimeras 337
25 Using Fluorescent Dyes for Fate Mapping， Lineage Analysis， and Axon Tracing in the Chick Embryo 351
26 Gene Transfer in Avian Embryos Using Replication-Competent Retroviruses
27 Electroporation in Avian Embryos
PART III. AMPHIBIAN EMBRYOS
28 An Overview of Xenopus Development
29 Mesoderm Induction Assays
30 Experimental Embryological Methods for Analysis of Neural Induction in the Amphibian 405
31 A Method for Generating Transgenic Frog Embryos 447
32 Axolotl/Newt 467
PART IV. ZEBRAFISH AND MEDAKA
33 The Zebrafish: An Overview of Its Early Development 483
34 Small-Scale Marker-Based Screening for Mutations in Zebrafish Development 493
35 Microinjection and Cell Transplantation in Zebrafish Embryos 513
36 Recent Advances in Meganuclease-and Transposon-Mediated Transgenesis of Medaka and Zebrafish 521
37 Retinoids in Nonmammalian Embryos
PART V. NONVERTEBRATE CHORDATES
38 Protochordates
PART VI. MOLECULAR TECHNIQUES
39 Subtractive Hybridization and Construction of cDNA Libraries 569
40 Differential Display of Eukaryotic mRNA 589
41 Using DNA Microarrays 605
42 Profiling Gene Transcription in the Developing Embryo: Microarray Analysis on Gene Chips 631
43 RT-PCR on Embryos Using Degenerate Oligonucleotide Primers 657
44 Single-Cell RT-PCR cDNA Subtraction 667
45 In-Situ Hybridization of Radioactive Riboprobes to RNA in Tissue Sections 675
46 In-Situ Hybridization and Immunohistochemistry in Whole Embryos 687
47 Wholemount In Situ Hybridization to Xenopus Embryos 697
48 Wholemount In Situ Hybridization to Amphioxus Embryos 703
49 In-Situ Hybridization to Sections (Nonradioactive) 707
50 Immunohistochemistry Using Polyester Wax 717
51 Immunohistochemistry on Whole Embryos 725
52 Whole Embryo Assays for Programmed Cell Death 729
53 Protein Techniques: Immunoprecipitation， In Vitro Kinase Assays， and Western Blotting 735
PART VII. MICROSCOPY AND PHOTOGRAPHY
54 Microscopy and Photomicrography Techniques
Index 773
