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Recombinant proteins from plants : methods and protocols / edited by Loic Faye and Veronique Gomord. — New York : Humana Press, c2009. – (58.17/M592/v.483) |
Contents
Contents
1. From Neanderthal to Nanobiotech: From Plant Potions to Pharming with Plant Factories
2. Cowpea Mosaic Virus-Based Systems for the Expression of Antigens and Antibodies in Plants
3. Transient Expression of Antibodies in Plants Using Syringe Agroinfiltration
4. Rapid System for Evaluating Bioproduction Capacity of Complex Pharmaceutical Proteins in Plants
5. Production and Localization of Recombinant Pharmaceuticals in Transgenic Seeds
6. Production of Antibody Fragments in Arabidopsis Seeds
7. Production of Plantibodies in Nicotiana Plants
8. Physcomitrella Patens: A Non-Vascular Plant for Recombinant Protein Production
9. Production of Recombinant Proteins in Suspension-Cultured Plant Cells
10. Chloroplast-Derived Vaccine Antigens and Biopharmaceuticals: Protocols for Expression， Purification， or Oral Delivery and Functional Evaluation
11. Protein Body Induction: A New Tool to Produce and Recover Recombinant Proteins in Plants
12. A Case Study for Plant-Made Pharmaceuticals Comparing Different Plant Expression and Production Systems 209
13. Glycosylation of Antibody Therapeutics: Optimisation for Purpose 223
14. N-Glycosylation of Plant Recombinant Pharmaceuticals 239
15. Companion Protease Inhibitors to Protect Recombinant Proteins in Transgenic Plant Extracts 265
16. Strategies for Improving Vaccine Antigens Expression in Transgenic Plants: Fusion to Carrier Sequences 275
17. Immunomodulation of Plant Function by In Vitro Selected Single-Chain Fv Intrabodies 289
18. On-Chip Detection of Low-Molecular-Weight Recombinant Proteins in Plant Crude Extracts by SELDI-TOF MS 313
19. Assessing the Risk of Undesirable Immunogenicity/Allergenicity of Plant-Derived Therapeutic Proteins 325
20. Biosafety， Risk Assessment and Regulation of Plant-Made Pharmaceuticals 341
