Transgenic wheat, barley and oats : production and characterization protocols / edited by Huw D. Jones and Peter R. Shewry. — New York : Humana Press, c2009. – (58.17/M592/v.478)

Contents

    CONTENTS
    
    PART I. INTRODUCTION
    1. Transgenic Wheat， Barley and Oats: Production and Characterization
    PART II. TRANSFORMATION AND REGENERATION
    2. Selection of Transformed Plants
    3. Reporter Genes
    4. Biolistics Transformation of Wheat
    5. Agrobacterium-Mediated Transformation of Bread and Durum Wheat Using Freshly Isolated Immature Embryos 93
    6. Floral Transformation of Wheat
    7. Highly Efficient Agrobacterium-Mediated Transformation of Wheat via In Planta Inoculation
    8. Barley Transformation Using Biolistic Techniques
    9. Barley Transformation Using Agrobacterium-Mediated Techniques
    10. Transformation of Oats and Its Application to Improving Osmotic Stress Tolerance
    PART III. GENE AND PROTEIN EXPRESSION
    11. Promoter Sequences for Defining Transgene Expression
    12. Down-Regulation of Gene Expression by RNA-Induced Gene Silencing
    PART IV. CHARACTERISATION OF TRANSGENIC PLANTS
    13. Gene Insertion Patterns and Sites
    14. Fluorescent In Situ Hybridization to Detect Transgene Integration into Plant Genomes
    15. Establishing Substantial Equivalence: Transcriptomics
    16. Establishing Substantial Equivalence: Proteomics
    17. Establishing Substantial Equivalence: Metabolomics
    18. Design and Management of Field Trials of Transgenic Cereals
    19. GM Risk Assessmcnt
    PART V. CONCLUSIONS
    20. Transgenic Wheat， Barley and Oats: Future Prospects