T cell protocols / edited by Gennaro De Libero. — 2nd ed. — Totowa, N.J. : Humana Press, c2009. – (58.17/M592/v.514)

Contents

    Contents
    
    1 Analysis of Frequency and Phenotype of Antigen-Specific T Cells
    2 B Cell Helper Assays
    3 transkingdom RNA Interference (tkRNAi): A Novel Method to Induce Therapeutic Gene Silencing
    4 Flow Cytometry and Cell Activation
    5 Investigating T Cells by Polychromatic Flow Cytometry
    6 Generation of Human T Cell Clones.
    7 Limiting Dilution Analysis of Antigen-Specific T Cells
    8 T Cell Epitope-Mapping by Cytokine Gene Expression Assay
    9 Cytokine Multiplex Immunoassay: Methodology and (Clinical) Applications 119
    10 Purification of the T Cell Antigen Receptor and Analysis by Blue-Native PAGE
    11 Non-Replicating Recombinant Vaccinia Virus Expressing CD80 to Enhance T-Cell Stimulation 151
    Index 163