Macromolecular drug delivery : methods and protocols / edited by Mattias Belting. — New York, N.Y. : Humana Press, c2009. – (58.17/M592/v.480)

Contents

    Contents
    
    Preface
    Contributors
    Color Plates
    1 Developments in Macromolecular Drug Delivery
    2 Synthetic vs. Natural/Biodegradable Polymers for Delivery of shRNA-Based Cancer Therapies
    3 siRNA and DNA Transfer to Cultured Cells 31
    4 Poly(13-amino esters): Procedures for Synthesis and Gene Delivery 53
    5 Systemic Delivery and Pre-clinical Evaluation of Nanoparticles Containing Antisense Oligonucleotides and siRNAs 65
    6 Peptide-Based Delivery of Steric-BIock PNA Oligonucleotides 85
    7 Characterizing Peptide-Mediated DNA Internalization in Human Cancer Cells 101
    8 Selection and Characterization of Antibodies from Phage Display Libraries Against Internalizing Membrane Antigens 113
    9 Artificial Membrane Models for the Study of Macromolecular Delivery .. 129
    10 Enhanced Delivery of Macromolecules into Cells by Electroendocytosis 141
    11 In Vitro Systems for Studying Epithelial Transport of Macromolecules 151
    12 Preparation of Macromolecule-Containing Dry Powders for Pulmonary Delivery 165
    13 Macromolecular Delivery Across the Blood-Brain Barrier 175
    14 Positron Emission Tomography (PET) and Macromolecular Delivery In Vivo 187
    Index 199