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新书资源(2009年2月)

Wnt signaling. v. 1, Pathway methods and mammalian models / edited by Elizabeth Vincan. — Totowa, N.J. : Humana Press, c2008. – (58.17/M592/v.468)

Contents

        Contents
    
    Preface
    Contents
    Contributors
    PART I. WNT SIGNALING PATHWAY METHODS
    SECTION A. CANONICAL WNT/FZD SIGNALING
    1. The Canonical Wnt/β-Catenin Signalling Pathway
    2. Isolation and Application of Bioactive Wnt Proteins
    3. Purification and Wnt-Inhibitory Activities of Secreted Frizzled-Related Proteins
    4. Measuring GSK3 Expression and Activity in Cells
    5. Inhibition of Glycogen Synthase Kinase-3
    6. Detection of Cytoplasmic and Nuclear Localization of Adenomatous Polyposis Coli (APC) Protein in Cells
    7. Detection of β-Catenin Localization by Immunohistochemistry
    8. Assaying β-Catenin/TCF Transcription with β-Catenin/TCF Transcription-Based Reporter Constructs
    9. Native Promoter Reporters Validate Transcriptional Targets
    SECTION B. NON-CANONICAL SIGNALING
    10. β-Catenin-Independent Wnt Pathways: Signals, Core Proteins, and Effectors 131
    11. Image Analysis of Calcium Release Dynamics 145
    12. Detecting PKC Phosphorylation as Part of the Wnt/Calcium Pathway in Cutaneous Melanoma 157
    13. Measuring CamKII Activity in Xenopus Embryos as a Read-out for Non-canonical Wnt Signaling 173
    14. Analysis of Wnt 7a-Stimulated JNK Activity and cJun Phosphorylation in Non-Small Cell Lung Cancer Cells
    15. ROCK Enzymatic Assay
    16. Detection of Planar Polarity Proteins in Mammalian Cochlea 207
    PART II. MAMMALIAN MODEL SYSTEMS FOR WNT/FZD FUNCTION
    17. Proteomic Analyses of Protein Complexes in the Wnt Pathway
    18. In Situ Hybridization to Evaluate the Expression of Wnt and Frizzled Genes in Mammalian Tissues
    19. Assaying Wnt5A-Mediated Invasion in Melanoma Cells
    20. Coculture Methodologies for the Study of Wnt Signals 255
    21. Analysis of Wnt/FZD-Mediated Signalling in a Cell Line Model of Colorectal Cancer Morphogenesis 263
    22. Analysing Tissue and Gene Function in Intestinal Organ Culture 275
    23. Genetics of Wnt Signaling During Early Mammalian Development
    24. Tissue-Specific Transgenic, Conditional Knockout and Knock-In Mice of Genes in the Canonical Wnt Signaling Pathway