![]() |
Molecular biomethods handbook / edited by John M. Walker, Ralph Rapley. — 2nd ed. — Totowa, NJ : Humana Press, c2008. – (58.178073/M718/2nd ed.) |
Contents
Content
Part A Nucleic Acid Methods
1 The Manipulation of Nucleic Acids: Basic Tools and Techniques
2 Restriction Enzymes: Tools in Clinical Research
3 Principles and Medical Applications of the Polymerase Chain Reaction
4 Probe Design, Production, and Applications
5 Southern Blotting as a Diagnostic Method
6 Capillary Electrophoresis of DNA: Biomedical Applications
7 Denaturing High-Performance Liquid Chromatography(DHPLC) for Nucleic Acid Analysis 89
8 Denaturing Gradient Gel Electrophoresis (DGGE) 107
9 Single Strand Conformation Polymorphism (SSCP) Analysis 117
10 Randomly Amplified Polymorphic DNA (RAPD): A Useful Tool for Genomic Characterization of Different Organisms 133
11 Quantification of mRNA Using Real Time RT-PCR: The SYBR Solution 149
12 Quantitative Analysis of DNA Sequences by PCR and Solid-Phase Minisequencing
13 Multiplex Amplifiable Probe Hybridization (MAPH) 179
14 Gene Expression Profiling
15 Comparative Genomic Hybridization in Clinical and Medical Research 213
16 Subtractive Hybridization 227
17 Fluorescence In Situ Hybridization 239
18 Quantitative Trait Locus Mapping to Identify Genes for Complex Traits in Mice 257
19 cDNA Microarrays 269
20 Mapping Techniques 291
21 Single Nucleotide Polymorphisms 311
22 Gene/Protein Sequence Analysis: A Compilation of Bioinformatic Tools 323
Part B Protein and Cell Methods
23 Protein Electrophoresis 351
24 Protein Blotting 365
25 Capillary Electrophoresis of Proteins 377
26 Autoradiography and Fluorography 397
27 Mass Spectrometry of Proteins and Peptides 411
28 Post-Translational Modifications of Proteins 427
29 Protein Microarray Technology 451
30 Protein-Protein Interactions 463
31 Glycoprotein Analysis 495
32 Solid-Phase Peptide Synthesis 515
33 Monoclonal Antibodies 547
34 Antibody Phage Display 563
35 Protein Engineering 587
36 Directed Protein Evolution 631
37 Enzyme Linked Immunosorbent Assay (ELISA) 657
38 Epitope Mapping: Identification of Antibody-Binding Sites on Protein Antigens 683
39 Quantum Dots 697
40 Ion-Exchange Chromatography 711
41 Size-Exclusion Chromatography 719
42 Hydrophobic Interaction Chromatography 731
43 Affinity Chromatography 741
44 High Performance Liquid Chromatography(HPLC) of Peptides and Proteins 767
45 Amino Acid Analysis 793
46 Surface Plasmon Resonance 809
47 Macromolecular Crystallography 821
48 Microchip Devices for Bioanalysis 851
49 Mammalian Cell Culture 861
50 Plant Tissue Culture 875
51 Stem Cells and Regenerative Medicine 905
52 Cryopreservation: Conservation of Bioresources at Ultra Low Temperatures 917
53 Magnetic Resonance Imaging
54 Electron Microscopy
55 Confocal Microscopy
56 Laser Microdissection 1027
57 Biomedical Uses of Flow Cytometry 1039
58 Immunomicroscopy
59 In Situ Hybridization
60 High Throughput Screening 1097
Index 1119