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Plant virology protocols : from viral sequence to protein function / Gary D. Foster ... [et al.]. — 2nd ed. — Totowa, NJ : Humana Press, c2008. – (58.17/M592/v.451) |
Contents
Contents
Preface
Contributors
Section 1 General Introduction
Chapter 1 Plant-Virus Interactions
Chapter 2 Role of Capsid Proteins
Chapter 3 Role of Plant Virus Movement Proteins 33
Chapter 4 Multiple Roles of Viral Replication Proteins in Plant RNA Virus Replication 55
Chapter 5 Role of Silencing Suppressor Proteins
Chapter 6 Role of Vector-Transmission Proteins
Section 2 Viral Nucleic Acid Sequence/Function Analysis
Chapter 7 In Vivo Analyses of Viral RNA Translation
Chapter 8 In Vitro Analysis of Translation Enhancers 113
Chapter 9 Identification of Plant Virus IRES 125
Chapter 10 Analysis of Geminivirus DNA Replication by 2-D Gel 135
Chapter 11 Begomoviruses: Molecular Cloning and Identification of Replication Origin 145
Chapter 12 Analysis of Viroid Replication 167
Chapter 13 Biochemical Analyses of the Interactions Between Viral Polymerases and RNAs 185
Chapter 14 In Situ Detection of Plant Viruses and Virus-Specific Products 201
Chapter 15 Detection of siRNAs and miRNAs
Chapter 16 Cloning of Short Interfering RNAs from Virus-Infected Plants 229
Chapter 17 Solution Structure Probing of RNA Structures
Chapter 18 RNA Encapsidation Assay 251
Section 3 Protein Analysis and Investigation of Protein Function
Chapter 19 Surface Plasmon Resonance Analysis of Interactions Between Replicase Proteins of Tomato Bushy Stunt Virus 267
Chapter 20 Biochemical Approaches for Characterizing RNA-Protein Complexes in Preparation for High Resolution Structure Analysis 279
Chapter 21 Probing Interactions Between Plant Virus Movement Proteins and Nucleic Acids 293
Chapter 22 Movement Profiles: A Tool for Quantitative Analysis of Cell-to-Cell Movement of Plant Viral Movement Proteins 317
Chapter 23 Analysis of siRNA-Suppressor of Gene Silencing Interactions 331
Chapter 24 Phosphorylation Analysis of Plant Viral Proteins 339
Chapter 25 Analysis of Interactions Between Viral Replicase Proteins and Plant Intracellular Membranes 361
Chapter 26 Membrane and Protein Dynamics in Virus-Infected Plant Ceils
Chapter 27 Site-Directed Mutagenesis of Whole Viral Genomes
Chapter 28 Viral Protein-Nucleic Acid Interaction: South (North)-Western Blot
Chapter 29 Protein-Protein Interactions: The Yeast Two-Hybrid System 421
Chapter 30 NMR Analysis of Viral Protein Structures 441
Chapter 31 Localization of Viral Proteins in Plant Cells: Protein Tagging 463
Section 4 Microscopy/GFP/Protein Tagging/Infections Clones and Other Such Tools
Chapter 32 Construction of Infectious Clones for RNA Viruses: TMV
Chapter 33 Construction of Infectious cDNA Clones for RNA Viruses: Turnip Crinkle Virus 491
Chapter 34 Construction of Infectious Clones for DNA Viruses: Mastreviruses
Chapter 35 Construction of Infectious Clones of Double-Stranded DNA Viruses of Plants Using Citrus Yellow Mosaic Virus as an Example 525
Chapter 36 Insertion of Introns: A Strategy to Facilitate Assembly of Infectious Full Length Clones 535
Chapter 37 Analysis of Cell-to-Cell and Long-Distance Movement of Apple Latent Spherical Virus in Infected Plants Using Green， Cyan， and Yellow Fluorescent Proteins 545
Chapter 38 Agroinoculation: A Simple Procedure for Systemic Infection of Plants with Viruses 555
Chapter 39 Geminivirus: Biolistic Inoculation and Molecular Diagnosis
Section 5 Genomics， Host Factors and Plant Based Studies
Chapter 40 Expression Microarrays in Plant-Virus Interaction 583
Chapter 41 Genome-Wide Screens for Identification of Host Factors in Viral Replication 615
Chapter 42 Phosphorylation of Movement Proteins by the Plasmodesmal-Associated Protein Kinase 625
Chapter 43 Virus-Induced Gene Silencing as a Tool to Identify Host Genes Affecting Viral Pathogenicity 641
Chapter 44 Yeast Two-Hybrid Assay to Identify Host-Virus Interactions 649
Index 673
