Protein engineering protocols / edited by Katja M. Arndt, Kristian M. Muller. — Totowa, N.J. : Humana Press, c2007. – (58.17/M592/v.352)

Contents

    Contents
    
    Preface
    Contributors
    PART I DESIGN AND COMPUTATIONAL STRATEGIES FOR PROTEIN FNGINEERING
    1 Combinatorial Protein Design Strategies Using Computational Methods
    2 Global Incorporation of Unnatural Amino Acids in Escherichia coli
    3 Considerations in the Design and Optimization of Coiled Coil Structures
    4 Calcium Indicators Based on Calmodulin-Fluorescent Protein Fusions
    5 Design and Synthesis of Artificial Zinc Finger Proteins
    6 Monobodies: Antibody Mimics Based on the Scaffold of the Fibronectin Type III Domain
    7 Engineering Site-Specific Endonucleases
    PART II EVOLUTIONARY STRATEGIES FOR PROTEIN ENGINEERING
    8 Protein Library Design and Screening: Working Out the Probabilities
    9 Protein Design by Binary Patterning of Polar and Nonpolar Amino Acids
    10 Versatile DNA Fragmentation and Directed Evolution With Nucleotide Exchange and Excision Technology
    11 Degenerate Oligonucleotide Gene Shuffling
    12 M13 Bacteriophage Coat Proteins Engineered for Improved Phage Display
    13 Ribosome-Inactivation Display System
    14 Compartmentalized Self-Replication: A Novel Method for the Directed Evolution of Polymerases and Other Enzymes
    15 Synthesis of Degenerated Libraries of the Ras-Binding Domain of Rafand Rapid Selection of Fast-Folding and Stable Clones With the Dihydrofolate Reductase Protein Fragment Complementation Assay
    16 A General Method of Terminal Truncation， Evolution， and Re-Elongation to Generate Enzymes of Enhanced Stability
    Index 305