In vitro transcription and translation protocols / edited by Guido Grandi. — 2nd ed. — Totowa, N.J. : Humana press, 2007. – (58.17/M592/v.375)

Contents

    Contents
    
    Preface
    Contributors
    PART I TECHNOLOGIES
    1 Energy Systems for ATP Regeneration in Cell-Free Protein Synthesis Reactions
    2 Continuous-Exchange Protein-Synthesizing Systems
    3 Cell-Free Production of Integral Membrane Proteins on a Preparative Scale
    4 SIMPLEX: Single-Molecule PCR-Linked In Vitro Expression: A Novel Method for High-Throughput Construction and Screening of Protein Libraries
    5 Methods for High-Throughput Materialization of Genetic Information Based on Wheat Germ Cell-Free Expression System
    6 Exogenous Protein Expression in Xenopus Oocytes: Basic Procedures
    PART II APPLICATIONS
    7 In Vitro Translation to Study HIV Protease Activity
    8 The Protein Truncation Test in Mutation Detection and Molecular Diagnosis
    9 Creation of Novel Enantioselective Lipases by SIMPLEX
    10 In Vitro Transcription and Translation Coupled to Two-Dimensional Electrophoresis for Bacterial Proteome Analysis
    11 In Vitro Screen of Bioinformatically Selected Bacillus anthracis Vaccine Candidates by Coupled Transcription， Translation， and Immunoprecipitation Analysis
    12 Functional Expression of Type 1 Rat GABA Transporter in Microinjected Xenopus laevis Oocytes
    13 Production of Protein for Nuclear Magnetic Resonance Study Using the Wheat Germ Cell-Free System