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Fundamental molecular biology / Lizabeth A. Allison. — Malden, MA : Blackwell, c2007.—(58.178/A438) |
Contents
Contents
Preface， xviii
1 The beginnings of motecutar biology， 1
1.1 Introduction
1.2 Historicat perspective
2 The structure of DNA， 13
2.1 Introduction
2.2 Primary structure: the components of nucteic acids
2.3 Significance of 5' and 3'
2.4 Nomenctature of nucteotides
2.5 The tength of RNA and DNA
2.6 Secondary structure of DNA
2.7 Unusuat DNA secondary structures
2.8 Tertiary structure of DNA
3 Genome organization: from nucteotides to chromatin， 37
3.1 Introduction
3.2 Eukaryotic genome
3.5 Bacteriophages and mammalian DNA viruses
3.6 0rganelle genomes: chloroplasts and mitochondria
3.7 RNA-based genomes
4 The versatitity of RNA， 54
4.1 Introduction
4.2 Secondary structure of RNA
4.3 Tertiary structure of RNA
4.4 Kinetics of RNA folding
4.5 RNA is involved in a wide range of cellular processes
4.6 Historical perspective: the discovery of RNA catalysis
4.7 Ribozymes catalyze a variety of chemical reactions
5 From gene to protein， 79
5.1 Introduction
5.2 The central dogma
5.3 The genetic code
5.4 Protein structure
5.5 Protein function
5.6 Protein folding and misfolding
6 DNA replication and tetomere maintenance， 108
6.1 Introduction
6.2 Historical perspective
6.3 DNA synthesis occurs from 5' --> 3'
6.4 DNA polymerases are the enzymes that catalyze DNA synthesis
6.5 Semidiscontinuous DNA replication
6.6 Nuclear DNA replication in eukaryotic cells
6.7 Replication of organelle DNA
6.8 Rotting circle replication
6.9 Tetomere maintenance: the rote of tetomerase in DNA replication， aging， and cancer
7 DNA repair and recombination， 152
7.1 Introduction
7.2 Types of mutations and their phenotypic consequences
7.3 General classes of DNA damage
7.4 Lesion bypass
7.5 Direct reversat of DNA damage
7.6 Repair of single base changes and structural distortions by removal of DNA damage
7.7 Double-strand break repair by removal of DNA damage
8 Recombinant DNA technotogy and motecutar ctoning， 180
8.1 Introduction
8.2 Historical perspective
8.3 Cutting and joining DNA
8.4 Molecular cloning
8.5 Constructing DNA libraries
8.6 Probes
8.7 Library screening
8.8 Expression libraries
8.9 Restriction mapping
8.10 Restriction fragment length potymorphism [RFLP}
8.11 DNA sequencing
9 Toots for anatyzing gene expression， 232
9.1 Introduction
9.2 Transient and stable transfection assays
9.3 Reporter genes
9.4 In vitro mutagenesis
9.5 Analysis at the level of gene transcription: RNA expression and localization
9.6 Analysis at the level of translation: protein expression and localization
9.7 Antisense technology
9.8 Analysis of DNA-protein interactions
9.9 Analysis of protein-protein interactions
9.10 Structural analysis of proteins
9.11 Model organisms
10 Transcription in prokaryotes， 278
10.1 Introduction
10.2 Transcription and translation are coupled in bacteria
10.3 Mechanism of transcription
10.4 Historical perspective， the Jacob-Monod operon model of gene regulation
10.5 Lactose (lac) operon regulation
10.6 Mode of action of transcriptional regulators
10.7 Control of gene expression by RNA
11 Transcription in eukaryotes， 312
11.1 Introduction
11.2 Overview of transcriptional regulation
11.3 Protein-coding gene regulatory elements
11.4 General [basatl transcription machinery
11.5 Transcription factors
11.6 Transcriptional coactivators and corepressors
11.7 Transcription complex assembly， the enhanceosome mode versus the "hit and run" model
11.8 Mechanism of RNA polymerase II transcription
11.9 Nuclear import and export of proteins
11.10 Regulated nuclear import and signal transduction pathways
12 Epigenetics and monoattetic gene expression， 392
12.1 Introduction
12.2 Epigenetic markers
12.3 Genomic imprinting
12.4 X chromosome inactivation
12.5 Phenotypic consequences of transposable elements
12.6 Epigenetic control of transposable elements
12.7 Allelic exclusion
13 RNA processing and post-transcriptionat gene regutation，452
13.1 Introduction
13.2 RNA splicing: historical perspective and overview
13.3 Group I and group II self-splicing introns
13.4 Archaet and nuclear transfer RNA introns
13.5 Cotranscriptional processing of nuclear pre-mRNA
13.6 Alternative splicing
13.7 Trans-splicing
13.8 RNAediting
13.9 Base modification guided by small nucteotar RNA molecules
13.10 Post-transcriptionat gone regulation by microRNA
13.11 RNA turnover in the nucleus and cytoplasm
14 The mechanism of translation， 512
14.1 Introduction
14.2 Ribosome structure and assembly
14.3 Aminoacyt-tRNA synthetases
14.4 Initiation of translation
14.5 Elongation
14.6 Termination
14.7 Translational and post-translational control
15 Genetically modified organisms: use in basic and appUed research， 545
15.1 Introduction
15.2 Transgenic mice
15.3 Gene-targeted mouse models
15.4 Other applications of transgenic animal technology
15.5 Cloning by nuclear transfer
15.6 Transgenic plants
16 Genome anatysis: DNA typing， genomics， and beyond， 581
16.1 Introduction
16.2 DNA typing
16.3 Genomics and beyond
16.4 The Human Genome Project
16.5 Other sequenced genomes
16.6 High-throughput analysis of gene function
16.7 Single nucleotide polymorphisms
17 Medical molecular biology， 618
17.1 Introduction
17.2 Molecular biology of cancer
17.3 Gene therapy
17.4 Genes and human behavior
