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A comparative methods approach to the study of oocytes and embryos / edited by Joel D. Richter. — New York : Oxford University Press, c1999.—(59.1151/C737) |
Contents
Contents
Contributors xi
Part I Methods for Studying Mouse Oocytes and Embryos
1 Mouse Oocyte Maturation， Fertilization， and Preimplantation Development in Vitro 3
John J. Eppig
2 Purification and Functional Analysis of Mouse Egg Zona Pellucida Glycoproteins 10
Eveline S. Litscher & Paul M. Wassarman
3 Methods for Analysis of Cytoplasmic Signal Transduction in Mammalian Eggs and Embryos 23
Christine M. Pauken & David G. Capco
4 Intracellular Ion Measurements in Single Eggs and Embryos Using Ion-Sensitive Fluorophores 39
Jay M. Baltz & Karen P. Phillips
5 Approaches to Study Translational Control in Mouse Oocytes 83
AndrO Stutz， Joachim Huarte， BOatrice Conne， & Jean-Dominique Vassalli
6 Identification of Genes and Processes Guiding the Transition between the Mammalian Gamete and Embryo 101
Bermseok Oh， Sue-Yun Hwang， Wilhelmine N. De Vries， Davor Solter， & Barbara B. Knowles
7 Approaches for the Analysis of Genomic Imprinting in Oocytes and Early Mouse Embryos 127
Angela J. Villar
8 Gene Expression in Mouse Embryos: Use of mRNA Differential Display 148
Richard M. Schultz
9 Use of Differential Display to Identify Gene Expression Differences in Male and Female Mouse Genital Ridges 157
Jay Brewster， Jill O'Moore， & William Crain
Part II Methods for Studying Xenopus Oocytes and Embryos
10 DNA Recombination and Repair in Xenopus Oocytes and Eggs: Substrate Design， Direct Microinjection， and Extract Preparation 173
Dana Carroll
11 DNA Replication and Chromatin Assembly Using Xenopus Eggs or Embryos 196
Sophie Menut， Jean-Marc Lemaitre， Alan Hair， & Marcel Mdchali
12 Isolation and Characterization of Masked mRNPs from Xenopus Oocytes 227
Mary T. Murray & Rolland Reinbold
13 Complications and Problems in the Assay of Transcription in Xenopus Oocytes， Eggs， and Embryos 241
Alan P. Wolffe
14 Analysis of Localized RNAs in Xenopus Oocytes 256
Malgorzata Kloc & Laurence D. Etkin
15 In Situ Hybridization Techniques with Xenopus Embryos 279
Thomas Hollemann， Frank Panitz， & Tomas Pieler
16 Visualizing Endogenous and Exogenous Proteins in Xenopus &ev&(and Other Organisms) 291
Timothy Carl & Michael W. Klymkowsky
17 Qualitative Analysis of Differential Gene Expression during Early Xenopus Embryogenesis by Whole-Mount In Situ Hybridization and RTPCR 316
L. Lynn McGrew， SteJan Hoppler， & Randall T. Moon
18 Studying the Function of Maternal mRNAs in Xenopus Embryos An Antisense Approach 341
M. V. Zuck， C. C. Wylie， & J. Heasman
19 Testing the Cell Fate Commitment of Single Blastomeres in Xenopus laevis 355
Sally A， Moody
Part III Methods for Studying Drosophila Oocytes and Embryos
20 Eggshell Assembly in Drosophila 385
Gall L. Waring
21 Cell Biological and Genetic Methods for Studying Drosophila Oogenesis 397
Denise J. Montell
22 Techniques for Analyzing Protein and RNA Distribution in Drosophila Ovaries and Embryos at Structural and Ultrastructural Resolution 426
Satoru Kobayashi， Reiko Amikura， Akira Nakamura， & Paul Lasko
23 Analysis of RNA Distribution during Drosophila Oogenesis Using Fluorescent In Situ Hybridization 446
Jolanta B. Glotzer & Anne Ephrussi
24 Assessment of Poly(A) Tail Lengths on Specific mRNAs during Development 457
Fernando J. Sall~s & Sidney Strickland
25 Three-Dimensional， Time-Lapse Microscopy of Drosophila Embryogenesis 471
Jonathan Minden
26 Anatomical Techniques for Analysis of Nervous System Development in the Drosophila Embryo 490
David Van Vactor & Casey Kopezynski
