Macromolecular crystallography protocols. v. 1, Preparation and crystallization of macromolecules / edited by Sylvie Doublie. — Totowa, New Jersey : Humana Press, c2007. – (58.17/M592/v.363)

Contents

    Contents
    
    1. A Generic Method for the Production of Recombinant Proteins in Escherichia coli Using a Dual Hexahistidine-Maltose-Binding Protein Affinity Tag
    2. Cloning， Production， and Purification of Proteins for a Medium-Scale Structural Genomics Project
    3. Baculoviral Expression of an Integral Membrane Protein for Structural Studies
    4. Protein Engineering
    5. Production of Selenomethionyl Proteins in Prokaryotic and Eukaryotic Expression Systems
    6. How to Use Dynamic Light Scattering to Improve the Likelihood of Growing Macromolecular Crystals
    7. Screening and Optimization Methods for Nonautomated Crystallization Laboratories
    8. Improving Marginal Crystals
    9. Optimization Techniques for Automation and High Throughput
    10. Three-Dimensional Crystallization of Membrane Proteins
    11. Crystallization of Protein-DNA Complexes
    12. Preparation and Crystallization of RNA
    13. Crystallization of RNA-Protein Complexes
    Index