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Cardiovascular proteomics : Methods and protocols / edited by Fernando Vivanco. — Totowa, New Jersey : Humana Press, c2007. – (58.17/M592/v.357) |
Contents
Contents
PART I. ANALYSIS OF THE HEART PROTEOME
1 Two-Dimensional Polyacrylamide Gel Electrophoresis for Cardiovascular Proteomics
2 Analyzing the Cardiac Muscle Proteome by Liquid Chromatography-Mass Spectrometry-Based Expression Proteomics 15
3 Two-Dimensional Differential Gel Electrophoresis of Rat Heart Proteins in Ischemia and Ischemia-Reperfusion 33
4 Analysis of Antihypertensive Drugs in the Heart of Animal Models: A Proteomic Approach 45
5 A Solubility Optimization Protocol For Two-Dimensional Gel Electrophoresis of Cardiac Tissue 59
6 A Method for Effective Depletion of Albumin From Cellular Extracts: Application to Human Myocardium 67
PART II. HEART SUBPROTEOMES
7 Subcellular Fractionation 73
8 Subfractionation of Heart Tissue: The "In Sequence" Myofilament Protein Extraction of Myocardial Tissue 87
9 Optimization of Cardiac Troponin I Pull-Down by IDM Affinity Beads and SELDI 91
10 Proteomic Analysis of the Subunit Composition of Complex I (NADH: Ubiquinone Oxidoreductase) From Bovine Heart Mitochondria 103
11 Identification of Targets of Phosphorylation in Heart Mitochondria 127
PART III. PROTEOM~CS OF HUMAN ATHEROMA PLAQUES
12 Characterization of the Human Atheroma Plaque Secretome by Proteomic Analysis 141
13 Characterization of HSP27 Phosphorylation Sites in Human Atherosclerotic Plaque Secretome 151
14 Western Array Analysis of Human Atherosclerotic Plaques 165
PART IV. THE PROTEOME OF CELLS OF THE CARDIOVASCULAR SYSTEM
15 The Proteome of Endothelial Cells 181
16 Proteomic Study of Caveolae and Rafts Isolated From Human Endothelial Ceils 199
17 Proteomic Identification of S-Nitrosylated Proteins in Endothelial Cells 215
18 The Proteome and Secretome of Human Arterial Smooth Muscle Cell 225
19 Real-Time In Vivo Proteomic Identification of Novel Kinase Substrates in Smooth Muscle 235
20 Proteomic Analysis of Vascular Smooth Muscle Cells Treated With Ouabain 253
21 Isolation and Culture of Adult Mouse Cardiac Myocytes 271
22 Proteomic Analysis of Foam Cells 297
23 Isolation of the Platelet Releasate 307
24 Enrichment of Phosphotyrosine Proteome of Human Platelets by Immunoprecipitation 313
25 Characterization of Circulating Human Monocytes by Proteomic Analysis 319
PART V. CARDIOVASCULAR BIOMARKERS IN PLASMA
26 Cardiovascular Biomarker Discovery by SELDI-TOF Mass Spectrometry 331
27 Detection of Biomarkers of Stroke Using SELDI-TOF 343
28 Depletion of High-Abundance Proteins in Plasma by Immunoaffinity Substraction for Two-Dimensional Difference Gel Electrophoresis Analysis 351
29 A Rapid， Economical， and Reproducible Method For Human Serum Delipidation and Albumin and IgG Removal For Proteomic Analysis 365
PART VI. BIOINFORMATICS: EMERGING TECHNOLOGIES
30 Mitoproteome: Human Heart Mitochondrial Protein Sequence Database 375
31 Techniques to Decipher Molecular Diversity by Phage Display 385
