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DNA microarrays. Part A, Array platforms & wet-bench protocols = DNA芯片. A辑, 芯片平台和湿实验方法 / edited by Alan Kimmel & Brian Oliver. — [Reprinted ed.]. — Beijing : Science Press ; [Amsterdam] : Elsevier, 2007.—(58.17435/C719(R)/v.410/pt.A) |
Contents
Contents
CONTRIBUTORS TO VOLUME 410
Section I Array Platforms
1. The Affymetrix GeneChip Platform: An Overview 3
2. The Agilent In Situ Synthesized Microarray Platform 28
3. Illumina Universal Bead Arrays 57
4. Microarray Oligonucleotide-probes 73
5. Automated Liquid Handling and High-Throughput Preparation of Polymerase Chain Reaction Amplified DNA for Microarray Fabrication 99
6. The Printing Process. Tips on Tips 121
7. Making and Using Spotted DNA Microarrays in an Academic Core Laboratory 135
8. Printing Your Own Inkier Microarrays 168
9. Peptide Nucleic Acid Microarrays Made with (S, S)-trans-Cyclopentane-Constrained Peptide Nucleic Acids 189
Section II Wet-Bench Protocols
10. Optimizing Experiment and Analysis Parameters for Spotted Microarrays 203
11. Sample Labeling.. An Overview 222
12. Genomic DNA as a General Cohybridization Standard for Ratiometric Microarrays 237
13. Analysis of Sequence Specificities of DNA-Binding Proteins with Protein Binding Microarrays 279
14. Microarray Analysis of RNA Processing and Modification 300
15. Mapping the Distribution of Chromatin Proteins by ChIP on Chip 316
16. DamID: Mapping of In Vivo Protein-Genome Interactions Using Tethered DNA Adenine Methyltransferase 342
17. Whole-Genome Genotyping 359
18. Mapping Drosophila Genomic Aberration Breakpoints with Comparative Genome Hybridization on Microarrays 377
19. Performing Quantitative Reverse-Transcribed Polymerase Chain Reaction Experiments 386
20. The Application of Tissue Microarrays in the Validation of Microarray Results 400
21. Mapping Histone Modifications by Nucleosome Immunoprecipitation 416
AUTHOR INDEX 431
SUBJECT INDEX 461