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Fluorescent energy transfer nucleic acid probes : designs and protocols / edited by Vladimir V. Didenko. — Totowa, New Jersey : Humana Press, c2006.—(58.17/M592/v.335) |
Contents
Contents
Preface
Contributors
Companion CD
PART I. DESIGN OF ENERGY TRANSFER PROBES
1 Selection of Fluorophore and Quencher Pairs for Fluorescent Nucleic Acid Hybridization Probes
2 Choosing Reporter-Quencher Pairs for Efficient Quenching Through Formation of Intramolecular Dimers
PART II.ENERGY TRANSFER PROBES FOR DNA AND RNA HYBRIDIZATION DETECTION AND MONITORING
3 Detection of DNA Hybridization Using Induced Fluorescence Resonance Energy Transfer
4 Detecting RNA/DNA Hybridization Using Double-Labeled Donor Probes With Enhanced Fluorescence Resonance Energy Transfer Signals
PART III. ENERGY TRANSFER PROBES FOR DNA BREAKS DETECTION AND DNA CLEAVAGE MONITORING
5 Oscillating Probe for Dual Detection of 5'PO4 and 5'OH DNA Breaks in Tissue Sections
6 Using Molecular Beacons for Sensitive Fluorescence Assays of the Enzymatic Cleavage of Nucleic Acids
7 A Continuous Assay for DNA Cleavage Using Molecular Break Lights
PART IV. MONITORING OF DNA SYNTHESIS ANO AMPLIFICATION USING ENERGY TRANSFER PROBES
Contents
8 Homogenous Detection of Nucleic Acids Using Self-Quenched Polymerase Chain Reaction Primers Labeled With a Single Fluorophore (LUXTM Primers)
9 Use of Self-Quenched， Fluorogenic LUXTM Primers for Gene Expression Profiling
10 TaqMan Reverse Transcriptase-Polymerase Chain Reaction Coupled With Capillary Electrophoresis for Quantification and Identification of bcr-abl Transcript Type
11 Quantitative TaqMan Assay for the Detection and Monitoring of Cytomegalovirus Infection in Organ Transplant Patients
12 Real-Time Detection and Quantification of Telomerase Activity Utilizing Energy Transfer Primers
PART V. DNA SEQUENCE ANALYSIS AND MUTATION DETECTION USING FLUORESCENCE ENERGY TRANSFER
13 Invader Assay for Single-Nucleotide Polymorphism Genotyping and Gene Copy Number Evaluation
14 Real-Time Quantitative Polymerase Chain Reaction Analysis of Mitochondrial DNA Point Mutation
15 Multiplex Single-Nucleotide Polymorphism Detection by Combinatorial Fluorescence Energy Transfer Tags and Molecular Affinity
16 High-Throughput Genotyping With Energy Transfer-Labeled Primers
Part VI. DETERMINATION OF Distance AND DNA FOLDING
17 Distance Determination in Protein-DNA Complexes Using Fluorescence Resonance Energy Transfer
18 Multi-Fluorophore Fluorescence Resonance Energy Transfer for Probing Nucleic Acids Structure and Folding
Part VII. DNA-Based BIOSENSORS UTILIZING ENERGY TRANSFER
19 Fluorescent DNAzyme Biosensors for Metal Ions Based on Catalytic Molecular Beacons
20 Fluorescent Energy Transfer Readout of an Aptazyme-Based Biosensor
21 Fluorescence Resonance Energy Transfer in the Studies of Guanine Quadruplexes
22 Solution-Phase Molecular-Scale Computation With Deoxyribozyme-Based Logic Gates and Fluorescent Readouts
Index 365
