MicroRNA protocols / edited by Shao-yan Ying. — Totowa, New Jersey : Humana Press, c2006.—(58.17/M592/v.342)

Contents

            Contents
    
    Preface
    Contributors
    1. The MicroRNA: Overview of the RNA Gene That Modulates Gene Functions
    2. Structure Analysis of MicroRNA Precursors
    3. MicroRNA Biogenesis: Isolation and Characterization of the Microprocessor Complex
    4. Recognition and Cleavage of Primary MicroRNA Transcripts
    5. Mouse Embryonic Stem Cells as a Model Genetic System to Dissect and Exploit the RNA Interference Machinery
    6. MicroRNAs and Messenger RNA Turnover
    7. Prediction of MicroRNA Targets
    8. Prediction of Human MicroRNA Targets
    9. Complications in Mammalian MicroRNA Target Prediction
    10. miRBase: The MicroRNA Sequence Database
    11. Methodologies for High-Throughput Expression Profiling of MicroRNAs
    12. In Situ Hybridization as a Tool to Study the Role of MicroRNAs in Plant Development
    13. Usefulness of the Luciferase Reporter System to Test the Efficacy of siRNA
    14. Cloning MicroRNAs From Mammalian Tissues
    15. Methods for Analyzing MicroRNA Expression and Function During Hematopoietic Lineage Differentiation
    16. Identifying MicroRNA Regulators of Cell Death in Drosophila
    17. MicroRNAs in Human Immunodeficiency Virus-1 Infection
    18. Cloning and Detection of HIV-l-Encoded MicroRNA
    19. Identification of Messenger RNAs and MicroRNAs Associated With Fragile X Mental Retardation Protein
    20. In Vitro Precursor MicroRNA Processing Assays Using Drosophila Schneider-2 Cell Lysates
    21. Downregulation of Human Cdc6 Protein Using a Lentivirus RNA Interference Expression Vector
    22. Gene Silencing In Vitro and In Vivo Using lntronic MicroRNAs
    23. Isolation and Identification of Gene-Specific MicroRNAs
    24. Transgene-Like Animal Models Using Intronic MicroRNAs
    25. Evolution of MicroRNAs
    26. Perspectives