Quantitative proteomics by mass spectrometry / edited by Salvatore Sechi. — Totowa, New Jersey : Humana Press, c2007.—(58.17/M592/v.359)

Contents

        Contents
    
    Preface v
    Contributors ix
    1 Acrylamide—A Cysteine Alkylating Reagent for Quantitative Proteomics 7
    2 Using Stable Isotope Tagging to Characterize Protein Complexes and to Detect Changes in Their Composition 17
    3 Stable Isotope Labeling by Amino Acids in Cell Culture for Quantitative Proteomics 37
    4 Quantitative Proteomics of Mouse B rain and Specific Protein．Interaction Studies Using Stable Isotope Labeling 53
    5 The Absolute Quantification Strategy：Application to Phosphorylation Profiling of Human Separase Serine 1126 77
    6 Quantification of Proteins and Metabolites by Mass Spectrometry Without Isotopic Labeling 87
    7 The Use of a Quantitative Cysteinyl-Peptide Enrichment Technology for High—Throughput Quantitative Proteomics 107
    8 An Isotope Coding Strategy for Proteomics Involving Both Amine and Carboxyl Group Labeling 725
    9 Proteolytic Labeling With 18O for Comparative Proteomics Studies：Preparation of 18O-Labeled Peptides and the 18O／16O Peptide Mixture 735
    10 Tandem Mass Spectrometrv in the Detection of Inborn Errors of Metabolism for Newborn Screening 743
    11 Absolute Quantification of Specific Proteins in Complex Mixtures Using Visible Isotope—Coded Affinity Tags 759
    12 Computational Analysis of Quantitative Proteomics Data Using Stable Isotope Labeling 177
    13 Quantitative Proteomic Analysis of Mammalian organisms Using Metabolically Labeled Tissues 191
    14 Quantitative Proteomic Analysis of Phosphotyrosine—Mediated Cellular Signaling Networks 203
    Index 213