Transmembrane signaling protocols /edited by Hydar Ali, Bodduluri Haribabu. Humana Press,2006. –(58.17/M592/v.332)

Contents

Contents:

1.       Transmembrane Signaling by G Protein-Coupled Receptors  3

2.       Crosstalk Coregulation Mechanisms of G Protein-Coupled Receptors and Receptor Tyrosine Kinases  51

3.       Protein-Protein Interactions in Signaling Cascades  79

4.       Biological Role of the CXCR4-SDF-1 Axis in Normal Human Hematopoietic Cells  103

5.       Functional Expression of CXCR4 in Saccharomyces cerevisiae in the Development of Powerful Tools for the Pharmacological Characterization of CXCP4  115

6.       Characterization of Constitutively Active Mutants of G Protein-Coupled Receptors  129

7.       G Protein-Coupled Receptor Dimerization and Signaling  141

8.       Real-Time Analysis of G Protein-Coupled Receptor Signaling in Live Cells  159

9.       Isolation of Membrane Rafts and Signaling Complexes  169

10.   Methods for the Study of Signaling Molecules in Membrane Lipid Rafts and Caveolae  181

11.   Bioluminescence Resonance Energy Transfer to Monitor Protein-Protein Interactions  195

12.   Identification of Interacting Proteins Using the Yeast Two-Hybrid Screen  211

13.   Analysis of  PDZ Domain Interactions Using Yeast Two-Hybrid and Coimmunoprecipitation Assays  233

14.   Clustering Assay for Studying the Interaction of Membrane Proteins With PDZ Domain Proteins  245

15.   Mammalian Cell Microinjection Assay to Study the Function of Rho Family Guanosine Triphosphatases  257

16.   Affinity-Based Assay of Rho Guanosine Triphosphatase Activation  269

17.   Assay of Phospholipase D Activity in Cell-Free Systems  281

18.   Reconstitution System Based on Cytosol-Depleted Cells to Study the Regulation of Phospholipased D  299

19.   Analysis of Global Gene Expression Profiles Activated by Chemoattractant Receptors  313

20.   Genetic Reconstitution of Bone Marrow for the Study of Signal Transduction Ex Vivo  331

21.   Proteomic Analysis of Human Neutrophils  343