Embryonic stem cell protocols. v. 1,Isolation and characterization /edited by Kursad Turksen. --Humana Press, 2006. –(58.17/M592/v.329)

Contents

    1. Isolation and Differentiation of Medaka Embryonic Stem Cells 3
    
    2. Maintenace of Chicken Embryonic Stem Cells In Vitro 17
    
    3. Derivation and Culture of Mouse Trophoblast Stem Cells In Vitro 35
    
    4. Derivation， Maintenance， and Induction of the Differentiation In Vitro of Equine Embryonic Stem Cells 59
    
    5. Derivation， Maintenance， and Induction of the Differentiation In Vitro of Equine Embryonic Stem Cells 59
    
    6. Generation and Characterization of Monkey Embryonic Stem Cells 81
    
    7. Derivation and Propagation of Embryonic Stem Cells in Serum-and Feeder-Free Culture 91
    
    8. Internal Standards in Differentiating Embryonic Stem Cells In Vitro 101
    
    9. Matrix Assembly， Cell Polarization ， and Cell Survival: Analysis of Peri-Implantation Development With Cultured Embryonic Stem Cells 113
    
    10. Phosphoinositides， Inositol Phosphates， and Phospholipase C in Embryonic Stem Cells 127
    
    11. Cripto Signaling in Differentiating Embryonic Stem Cells 151
    
    12. The Use of Embryonic Stem Cells to Study Hedgehog Signaling 171
    
    13. Transfection and Promoter Analysis in Embryonic Stem Cells 187
    
    14. SAGE Analysis to Identify Embryonic Stem Cell-Predominant Transcripts 195
    
    15. Utilization of Digital Differential Display to Identify Novel Targets of Oct3/4 223
    
    16. Gene Silencing Using RNA Interference in Embryonic Stem Cells 233
    
    17. Efficient Transfer of HSV-1Amplicon Vectors Into Embryonic Stem Cells and Their Derivatives 265
    
    18. Lentiviral Vector-Mediated Gene Transfer in Embryonic Stem Cells 273
    
    19. Use of the Cytomegalovirus Promoter for Transient and Stable Transgene Expression in Mouse Embryonic Stem Cells 283
    
    20. Use of Simian Immunodeficiency Virus Vectors for Simian Embryonic Stem Cells 295
    
    21. Generation of Green Fluorescent Protein-Expressing Monkey Embryonic Stem Cells 305
    
    22. DNA Damage Response and Mutagenesis in Mouse Embryonic Stem Cells 313
    
    23. Ultraviolet-Induced Apoptosis in Embryonic Stem Cells in Vitro 327
    
    24. Use of Differentiating Embryonic Stem Cells in Pharmacological Studies 341
    
    25. Embryonic Stem Cells as a Source of Differentiated Neural Cells for Pharmacological Screens 353
    
    26. Use of Murine Embryonic Stem Cells in Embryotoxicity Assays: The Embryonic Stem Cell Test 371
    
    27. Use of Chemical Mutagenesis in Mouse Embryonic Stem Cells 397
    
    28. Nuclear Reprogramming of Somatic Nucleus Hybridized With Embryonic Stem Cells by Electrofusion 411
    
    29. Methylation in Embryonic Stem Cells in Vitro 421
    
    30. Identification of Genes Involved in Tumor-Like Properties of Embryonic Stem Cells 449
    
    31. In Vivo Tumor Formation From Primate Embryonic Stem Cells 459
    
    32. Directed Differentiation and Characterization of Genetically Modified Embryonic Stem Cells for Therapy 471
    
    33. Use of Differentiating Embryonic Stem Cells in the Parkinsonian Mouse Model 485