Photosynthesis research protocols / edited by Robert Carpentier. -- (Methods in molecular biology ; v. 274). -- Humana Press, 2004. -- (58.17/M592/v.274)

Contents:

1. Fractionation of Thylakoid Membranes Into Grana and Stroma Thylakoids  1

2. Isolation of Photosystem I Particles From Spinach  11

3. Rapid Isolation and Purification of Photosystme I ChlorophyII-Binding Protein From Chlamydomonas Reinhardtii  19

4. Isolation of Photosystem II-Enriched Membranes and the Oxygen-Evolving Complex Subunit Proteins From Higher Plans  29

5. Isolation of Functional Photosystems II Core Particles From the Cyanobacterium Synechocystis sp. PCC 6803  37

6. Isolation of Photosystme I Reaction Center Preparation From Spinach  49

7. Isolation of Photosystme II Reaction Center complexes From Plants  53

8. Isolation of Photosystem I Reaction Center Subunit Polypeptides From Spinach  63

9. Purification and Crystallization of the Cytochrome b6f Complex in Oxygenic Photosynthesis  67

10. Purification of Plastocyanin and Cytochrome c6 From Plants, Green algae, and Cyanobacteria  79

11. Preparation of Native and Recombinant Light-Harvesting ChlorophyII-a/b Complex  93

12. Isolation and Characterization of Lamellar Aggregates of LHCII and LHCII-Lipid Macro-Assemblies With Light-Inducible Structural Transitions  105

13. Separation, Purification, and Characterization of Polypeptide Composition of Subcomplexes of the Main Light-Harvesting ChlorophyII a/b-Protein Complex of Photosystem II  115

14. Isolation of CP43 and CP47 Photosystme II Proximal Antenna Complexes From Plants  129

15. The Determination and Quantification of Photosynthetic Pigments by Reverse Phase High-Performance Liquid Chromatography, thin-Layer Chromatography, and Spectrophotometry  137

16. Isolation and Identification of Chloroplast Lipids  149

17. DNA Adducts With ChlorophyII and Chlorophllin As Antimutagenic  Agents: synthesis, Stability, and Structural Features  159

18. Incorporation and Analysis of LHCII in Model Systems  173

19. Photosystem II Reconstitution Into Proteoliposomes: Structure-Function Characterization  183

20. Extraction of the Functional Managanese and Calcium From Photosystem II  205

    ...   ...