Cell Migration in Inflammation and Immunity L Methods and Protocols / edited by Daniele D'Ambrosio and Francxesco Sinigaglia. -- (Methods in molecular Biology ; v.239). -- Humana Press, 2004

Contents

索书号:58.17/M592/v.239

1. Chemotaxis and Interaction with Vascular or Lymphatic Endothelium  1

2. Analysis of Integrin-Dependent Rapid Adhesion Under Laminar-Flow Conditions  17

3. Posttranslational Processing of Chemokines  27

4. Chemotactic Profiling of Lymphocyte Subpopulations  45

5. Measurement of the Levels of Polymerized Actin (F-Actin) in Chemokine-Stimulated Lymphocytes and GFP-Coupled cDNA Transfected Lymphoid Cells by Flow Cytometry  53

6. Evaluation of Rho Family Small G-Protein Activity Induced by Integrin Ligation of Human Leukocytes  69

7. Reconstructing Leukocyte Migration in 3D Extracellular Matrix by Time-Lapse Videomicroscopy and Computer-Assisted Tracking  77

8. Analyzing Chemotaxis Using Dictyostelium discoideum as a Model System  91

9. Conditional Transgenic Models to Study Chemokine Biology  105

10. Intraital Microscopy as a Tool for Studying Recruitment and Chemotaxis  123

11. Tracking Antigen-Specific Lymphocytes in Vivo  133

12. Analysis of Homing-Receptor Expression on Infiltrating Leukocytes in Disease States  147

13. Interaction of Viral Chemokine Inhibitors with Chemokines  167

14. Discovery of Small-Molecule Antagonists of Chemokine Receptors: Screening Strategy and Assays  181

15. Visualization and Analysis of Adhesive Events in Brain Microvessels by Using Intravital Microscopy  189

16. Animal Models to Study Chemokine Receptor Function Vivo: Mouse Models of Allergic Airway Inflammation  199

17. Assessing the Role of Multiple Phosphoinositide 3-Kinases in Chemokine Signaling: Use of Dominant Negative Mutants Controlled by a Tetracycline-Regulated Gene Expression Systems  211

18. In Vitol and Vivo Models to Study Chemokine Regulation of angiogenesis  223

19. Real Time Vitro Assay for Studying Chemoattractant-Triggered Leukocyte Transendothelial Migration Under Physiological Flow Conditions  233

20. Generation of Monoclonal Antibodies Against Chemokine Receptors  243

21. Detection of High-Affinity α4-Integrin Upon Leukocyte Stimulation by Chemoattractants of Chemokines  261