Protein NMR Techniques /edited by A. Kristina Downing. -- 2nd ed. -- (Methods ijn molecular biology ; v. 278). -- 2nd ed. -- Humana Press, 2004

Contents

索书号：58.17/M592/v.278

1. Screening and Optimizing Protein Production in E. coli  1

2. Isotopic Labeling of Recombinant Proteins From the Methylotrophic Yeast Pichia pastoris  17

3. Perdeuteration/Site-Specific Protonation Approaches  for High-Molecular-Weight Proteins  35

4. Segmental Isotopic Labeling for Structural biological Applications of NMR  47

5. TROSY-Based Correlation and NOE Spectroscopy for NMR Structural Studies of Large Proteins  57

6. Media for Studies of Partially Aligned States  79

7. Residual Dipolar Couplings in Protein Structure Determination  89

8. Projection Angle Restraints for Studying Structure and Dynamics of Biomolecules  107

9. Characterizing Domain Interfaces by NMR  123

10. Characterization of the Overall Rotational Diffusion of a Protein From 15N Relaxation Measurements and Hydrodynamic Calculations  139

11. TROSY-Based NMR Experiments for the Study of Macromolecular Dynamics and Hydrogen Bonding  161

12. Measurement of Intermediate Exchange Phenomena  185

13. NMR Studies of Partially Folded Molten-Globule States  233

14. Structure Determination of Protein Complexes by NMR  255

15. NMR Studies of Protein-Nucleic Acid Interactions  289

16. Using NMRView to Visualize and Analysis the NMR Spectra of Macromolecules  313

17. Automated NMR Structure Calculation With CYANA  353

18. NOE Assignment With ARIA 2.0: The Nuts and Bolts  379

19. Membrane Protein Structure Determination Using Solid-State NMR  403