B Cell Protocols / edited by Hua Gu, Klaus Rajewsky. -- (Methods in molecular biology ; v. 271). -- Humana press, 2004

Contents

 索书号：58.17/M592/v.271

1. Characterization of B Lymphopoiesis in Mouse Bone Marrow and Spleen  1

2. Characterization of B-Cell Maturation in the Peripheral Immune System  25

3. Identification of B-Cell Subsets: An Exposition of 11-Color (Hi-D) FACS Methods  37

4. Analysis of B-Cell Life-Span and Homeostasis  59

5. Differentiation of B Lymphocytes From Hematopoietic Stem Cells  67

6. Analysis of Lymphocyte Development and function Using the RAG-Deficient Blastocyst Complementation System  77

7. Conditional Gene Mutagenesis in B-Lineage Cells  91

8. Analysis of the Germinal Center Reaction and In Vivo Long-Lived Plasma Cells  111

9. T-Cell-Dependent Immune Responses, Germinal Center Development, and the Analysis of V-Gene Sequences  127

10. Introduction of Genes Into Primary Murine Splenic B Cells Using Retrovirus Vectors  139

11. Immunoglobulin Class Switching: In Vitro Induction and Analysis  149

12. In Vitro and In Vivo Assays of B-Lymphocyte Migration  161

13. Analysis of Antigen-Specific B-Cell Memory Directly Ex Vivo  173

14. B-Cell Signal Transduction: Tyrosine Phosphorylation, Kinase Activity, and Calcium Mobilization  189

15. Isolation of Lipid Rafts From B Lymphocytes  213

16. Molecular Single-Cell PCR analysis of Rearranged Immunoglobulin Genes As a Tool to Determaine the Clonal Composition of Normal and Malignant Human B Cells  225

17. Analysis of B-Cell Immune Tolerance Induction Using Transgenic Mice  239

18. Analysis of B-Cell Signaling Using DT40 B-Cell Line  261

19.B-Cell Development and Pre-B-1 Cell Plasticity In Vitro  271