Phage display : a practical approach /edited by Tim Clackson and Henry B. Lowman. -- (Practical approach series). -- Oxford University press, 2004

Contents

索书号：63.383/P532

1. Introduction to phage biology and phage display  1

2. Constructing phage display libraries by oligonucleotide-directed mutagenesis  27

3. In vitro DNA recombination  43

4. Phage selection strategies for improved affinity and specificity of proteins and peptides  61

5.Rapid screening of phage displayed protein binding affinities by phage ELISA  85

6. Identification of novel ligands for receptors using recombinant peptide libraries  95

7. Substrate phage display  117

8. Protease-based selection of stably folded proteins and protein domains from phage display libraries  135

9. Phage display of zinc fingers and other nucleic acid-binding motifs  155

10. In vivo and ex vivo selections using phage-displayed libraries  171

11.Screening phage libraries with sera  193

12.Interaction cloning using cDNA libraries displayed on phage  223

13.Phage antibody libraries  243

14.Affinity maturation of phage antibodies  289