Gene transfer and expression in mammalian cells / edited by Savvas C. Makrides. -- New Comprehensive Biochemistry ; v.38). -- Elsevier, 2003

Contents

索书号： 58.173/N532/v.38

1. Why choose mammalian cells for protein production?  1

2.Vectors for gene expression in mammalian cells  9

3.Virus-based vectors for gene expression in mammalian cells: Herpes simplex virus  27

4.Methods for DNA introduction into mammalian cells  265

5.Lipid reagents for DNA transfer into mammalian cells  279

6.Reporter genes for monitoring gene expression in mammalian cells  291

7.Gene transfer and gene amplification in mammalian cells  309

8.Co-transfer of multiple plasmids/viruses as an attractive method to introduce several genes in mammalian cells  337

9.Optimization of plasmid backbone for gene expression in mammalian cells  349

10.Use of scaffold/matrix-atta 

11.Chromatin insulators and position effects  381

12.Locus control regions  397

13.Protein synthesis ,

14.Pathways and functions of mammalian protein glycosylation  433

15.Metabolic engineering of  mammalian cells for higher protein yield  457

16.Translational regulation in mammalian cells  471

17.Pathways of mammalian messenger RNA degradation  495

18.Pathways of mammalian protein degradation  513

19.Stabilization of proteasomal substrates by viral repeats  535

20.Architecture and utilization of highly expressed genomic sites  551

21.Intracellular targeting of antibodies in mammalian cells.  573

22.Inducible gene expression in mammalian cells.  589

23.Protein production by large-scale mammalian cell culture  605

24.Protein production in transgenic animals  625

25.Strategies for the purification of recombinant proteins  641