Protein-Ligand interactions : methods and applications / edited by G. Ulrich Nienhaus. -- (Methods in molecular biology ; v. 305). -- Humana Press, 2005. -- (58.17/M592/v.305)

Contents

Contents:

1. Isothermal Titration Calorimetry  1

2. Direcr Optical Detection of Protein-Ligand Interactions  17

3. Label-Free Detection of Protein-Ligand Interactions by the Quartz Crystal Microbalance  47

4. Measurement of Solvent Accessibility at Protein-Protein Interfaces  65

5. Hydrophobic Interaction Chromatography: harmessing Multivalent Protein=-surface Interactions for Purification Procedures  81

6. Sedimentation Velocity Method in the analytical Ultracentrifuge for the Study of Protein-Protein Interactions  101

7. Protein-Ligand Interaction Probed by Time-Resolved Crystallography  115

8, X-Ray Crytallography of Protein-Ligand Interactions  155

9. combined Use of XAFS and Crystallography for Studying Protein-Ligand Interactions in Metalloproteins  167

10. NMR Studies of Protein-Ligand Interactions  197

11. Probing Heme Protein_Ligand Interactions by UV/Visible Absorption Specytoscopy  215

12. Ultrafast Time-Resolved IR Studies of Protein-Ligand Interactions  243

13. Monitoring Protein-Ligand Interactions by Time-Resolved FTIR Difference Spectroscopy  261

14. Protein in Motion: Resonance Raman Spectroscopy as a Proble of Functional Intermediates  287

15. fluorescence Polarization/anisotropy approaches to Study Protein-Ligand Interactions: Effects of Errors and Uncertainties  301

16. Ligand Binding With Stopped-Flow Rapid Mixing  323

17. Circular dichrosim Spectroscopy for the Study f Protein-Ligand Interactions  343

18. High-throughput Screening of Interactions Between G Protein-Coupled Receptors and Ligands Using Confocal Optics Microscopy  365

19. single-Molecule Study of Protein-Protein and Protein-DNA Interaction dynamics  385

20. application of Fluorescence Correlation Spectroscopy to Hapten-Antibody Binding  415

21. Atomic Force Microscopy Measurements of Protein-Ligand Interactions on Living Cells  439

22. computer simulation of Protein-Ligand Interactions: challenges and applications  451

23. Force Probe Milecular dynamics simulations  493

24. Study of Ligand-Protein Interactions by Means of Density functional theory and First-Principles Molecular Dynamics  517