Chemical Genomics : reviews and protocols / edited by D. Zanders. -- (Methods in molecular biology ; v. 310). -- Humana Press, 2005. -- (58.17/M592/v.310_

Contents

Contents:

1. Introduction to Chemical Genomics  3

2. Chemistry for Chemical Genomics  11

3. Computer-Aided Design of Small Molecules for Chemical Genomics  25

4. Design, Synthesis, and Screening of Biomimetic Ligands for Affinity chromatography  43

5. The Role and application of In silico docking in Chemical Genomics Research  63

6. synthesis of complex Carbohydrates and Glyconjugates: enzymatic Syntjesis of Globotetraose Using B-1, 3-N-Acetylgalactosaminyltransferase LgtD From Haemophilus infuenzae Strain Rd  93

7. High-Throughput Cloning for Proteomics Research  107

8. Screening for the Expression of Solyble Recombinant Protein in Escherichia coli  115

9. High-Throughput Pruification of Hexahistidine-Tagged Proteins Expressed in E. coli  123

10. The Wheat Germ Cell-Free Expression system: Methods for High-Throughput Materalization of Genetic Information  131

11. Advances in Genome-wide Protein Expression Using the Wheat Germ Cell-Free System  145

12. Production of Proteins for NMR Studies Using the Wheat Germ Cell-Free System  169

13. Adenoviral Expression of Reproter Proteins for High-Throughput Cell-Based Screening  187

14. Fabrication of Protein function Microarrays for Systems-Oriented Proteomic analysis n 197

15. Peptide and Small-Molecule Microarrays  217

16. Peptide Mass fingerprinting: Protein Identification Using MALDI-TOF Mass Spectrometry  227

17. A Practical Protocol for Carbohydrate Microarrays  241

18. Development of a Yeast Two-Hybrid Screen for Selection of Human Ras-Raf Protein Interaction Inhibitors  253