Methods in Cell Biology :v.71, Neurons: Methods and Applications for the Cell Biologist / edited by Peter J. Hollenbeck and James R. Bamburg. -- Academic Press, 2003 |
Contents
索书号: 58.1574/M592/V.71 Contents: 1. Comparing the Properties of Neuronal Culture Systems: A Shopping Guide for the Cell Biologist 2 2. Growing and Working with Peripheral Neurons 18 3. Dissection and Culturing of Chick Ciliary Ganglion Neurons: A System Well Suited to Synaptic Study 38 4.The Culture of Chick Forebrain Neurons 52 5.Growing and Working with Spinal Motor Neurons 68 6.Avian Purkinje Neuronal Cultures: Extrinsic Control of Morphology bu Cell Type and Glutamate 90 7.Culturing Hippocampal and Cortical Neurons 112 8. Working with Xenopus Spinal Neurons in Live Cell Culture 130 9.Culturing Neurons from the Snail Helisoma 158 10. The Tibial-1 Pioneer Pathway: An in Vivo Model for Neuronal Outgrowth and Guidance 172 11.Techniques to Dissect Cellular and Subcellular Function in the Drosophila Nervous System 196 12.PC12 Cells as a Model Studies of Regulated Secretion in Neuronal and Endocrine Cells 268 13.B35 Neuroblastoma Cells: An Easily Transfected, Cultured Cell Model of Central Nervous Sytem Neurons 288 14.Live-Cell Imaging of Slow Axonal Transport in Cultured Neurons 306 15.Making Proteins into Drugs: Assisted Delivery of Proteins and Peptides into Living Neurons 326 16.Tranfection of Primary Central and Peripheral Nervous System Neurons 340 17.Biolistic Transfection 354 18.Expression of Transgenes in Primary Neurons from Chick Peripheral and Central Nervous Systems by Retroviral Infection of Early Embryos 370 19.Production and Use of Replication-Deficient Adenovirus for Transgene Expression in Neurons 388
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