Epidermal growth factor :bmethods and protocols /edited by Tarun B. Patel, Paul J. Bertics. -- Humana Press, 2006. -- (58.17/M592/v.327)

Contents

Contents:

1.       A historical perspective of the egf receptor and related systems  1

2.       Purification and assay of kinase-active EGF receptor from mammalian cells by immunoaffinity chromatography  25

3.       Soluble epidermal growth factor receptor acridinium-linked immunosorbent assay  39

4.       Analysis of EGF receptor interactions with the a subunit of the stimulatory GTP binding protein of adenylyl cyclase, G  49

5.       Regulator of epidermal growth factor signaling: sprouty  61

6.       Dissecting the epidermal growth factor receptor signal transactivation pathway  85

7.       A sensitive method to monitor ectodomain shedding of ligands of the epidermal growth factor receptor  99

8.       in vitro and in vivo assays of monoubiquitination of receptor tyrosine kinases  115

9.       Assays to monitor degradation of the EGF Receptor  131

10.   Assessment of Degradation and ubiquitination of CXCR4, a GPCFR Regulated by EGFR Family members  139

11.   Epithelial cell migration in response to epidermal growth factor  147

12.   Motility signaled from the EGF Receptor and related systems  159

13.   Methods for determining the proliferation of cells in response to EGFR ligands  179

14.   Clinical advancement of EGFR inhibitors in cancer therapy  189