Nuclear reprogramming :bmethods and protocols /edited by Steve Pells. -- Humana Press, 2006. – (58.17/M592/v. 325)

Contents

Contents:

1.       Nuclear transplantation xenopus  1

2.       Nuclear transfer in sheep  11

3.       Protocols for nuclear transfer in mice  25

4.       Isolation of stromal stem cells from human adipose tissue Nuclear reprogramming by cell fusion  47

5.       Nuclear reprogramming by cell fusion  47

6.       Polyethylence Glycol-mediated cell fusion  59

7.       Epigenetic reprogramming of somatic genomes by electrofusion with embryonic stem cells  67

8.       Quantification of cell fusion by flow cytometry  81

9.       Modulation of Cell fate using nuclear and cytoplasmic extract  99

10.   transgenic systems in nuclear reprogramming  115

11.   Using immunofluorescence to observe methylation changes in mammalian preimplantation embryos  129

12.   Observing S-phase dynamics of histone modifications with fluorescently labeled antibodies  139

13.   Quantitative analysis of telomerase activity and telomere length in domestic animal clones  149

14.   Pluripotency: Capacity for in vitro differntiation of undifferentiated embryonic stem cells  181

15.   Staining embryonic stem cells using monoclonal antibodies to stage-specific embryonic antigens  207

16.   Analysis of the nucleolar compartment of the nucleus as an indicator of nuclear reprogramming after nuclear transfer  225

17.   Methylation-sensitive polymerase chain reaction  239

18.   Analysis of DNA methylation profiles in preimplantation embryos using bisulfite mutagenesis  251

19.   Chromatin immunoprecipitation assay for mammalian tissues  261

20.   Histone modifications and transcription factor binding on chromatin: Chip-pcr assays  273

21.   In vivo genomic footprinting using LM_Pcr methods  285

22.   Analyzing histone modification using crosslinked chromatin treated with micrococcal nuclease  315