Yeast Protocols /edited by Wei Xiao. --Humana Press, 2006. --(58.17/M592/v.313)

Contents

Contents:

1. Basic Investigations in Saccharomyces cerevisiae  1

2. Isolation of Nucleic Acids  15

3. Purification of Yeast Peroxisomes  21

4. Isolation of Yeast Plasma Mebranes  27

5. Isolation of Yeast Mitochondria  33

6. Extraction of Yeast Lipids  41

7. Two-Dimensional Gel Electrophoresis of Total Yeast Proteins  47

8. Pulsed-Field Gel Electrophoresis of Budding Yeast Chromosomes  65

9. Analysis of Yeast Lipids  75

10. Yeast Fluorescence Microscopy  85

11. Intracellular Expression of Recombinant Antibody Fluorescent Protein

      Fusions for Localization of Target Antigens in Schizosaccharomyces

      pombe  97

12. Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method  107

13. Mutagenesis  121

14. Gene Disruption in the Budding Yeast Saccharomyces cerevisiae  129

15. Inducible Degron and its Application to Creating Conditional mutants  145

16. Synthetic Lethal Screen  161

17. Synthetic Genetic Array Analysis in Saccharomyces cervisiae  171

18. Two-Dimensional Agarose Gel Analysis of DNA Replication

      intermediates  193

19. Chromatin Assembly in a Crude Fraction From Yeast Cells  209

20. Chromatin Immunoprecipitation to Study Protein-DNA Interactions in

      Budding Yeast  225 

      ... ...